Detailed information of TA system TA040158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2414080..2414718
• Replicon: chromosome
• Accession: NZ_AP025220
• Organism: Escherichia coli strain 2017.11.01CC

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LPH31_RS11725
• Protein ID: WP_000813794.1
• Coordinates: 2414542..2414718 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LPH31_RS11720
• Protein ID: WP_001270286.1
• Coordinates: 2414080..2414496 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH31_RS11700 (2409232)	2409232..2410173	-	942	WP_001251313.1	ABC transporter permease	-
LPH31_RS11705 (2410174)	2410174..2411187	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
LPH31_RS11710 (2411205)	2411205..2412350	-	1146	WP_072778914.1	ABC transporter substrate-binding protein	-
LPH31_RS11715 (2412595)	2412595..2414001	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
LPH31_RS11720 (2414080)	2414080..2414496	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LPH31_RS11725 (2414542)	2414542..2414718	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LPH31_RS11730 (2414940)	2414940..2415170	+	231	WP_000494244.1	YncJ family protein	-
LPH31_RS11735 (2415262)	2415262..2417223	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LPH31_RS11740 (2417296)	2417296..2417832	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
LPH31_RS11745 (2417924)	2417924..2419099	+	1176	WP_001236251.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2419139..2420404	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T40158 WP_000813794.1 NZ_AP025220:c2414718-2414542 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T40158 NZ_AP025220:c2414718-2414542 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT40158 WP_001270286.1 NZ_AP025220:c2414496-2414080 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT40158 NZ_AP025220:c2414496-2414080 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB