Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 892778..893432 Replicon chromosome
Accession NZ_AP025220
Organism Escherichia coli strain 2017.11.01CC

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LPH31_RS04360 Protein ID WP_000244781.1
Coordinates 893025..893432 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LPH31_RS04355 Protein ID WP_000354046.1
Coordinates 892778..893044 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH31_RS04330 (888592) 888592..888903 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
LPH31_RS04335 (889067) 889067..889726 + 660 WP_000250274.1 hemolysin III family protein -
LPH31_RS04340 (889757) 889757..890224 - 468 WP_032303738.1 hypothetical protein -
LPH31_RS04345 (890221) 890221..891519 - 1299 WP_050486624.1 hypothetical protein -
LPH31_RS04350 (891546) 891546..892526 - 981 WP_032303737.1 tRNA-modifying protein YgfZ -
LPH31_RS04355 (892778) 892778..893044 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LPH31_RS04360 (893025) 893025..893432 + 408 WP_000244781.1 protein YgfX Toxin
LPH31_RS04365 (893472) 893472..893993 - 522 WP_032303736.1 flavodoxin FldB -
LPH31_RS04370 (894105) 894105..895001 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LPH31_RS04375 (895026) 895026..895736 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LPH31_RS04380 (895742) 895742..897475 + 1734 WP_077516661.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T40147 WP_000244781.1 NZ_AP025220:893025-893432 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T40147 NZ_AP025220:893025-893432 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCCCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTACGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT40147 WP_000354046.1 NZ_AP025220:892778-893044 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT40147 NZ_AP025220:892778-893044 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References