Detailed information of TA system TA040143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 39830..40431
• Replicon: plasmid p20170902CC-2
• Accession: NZ_AP025216
• Organism: Escherichia coli strain 2017.09.02CC

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LPH32_RS23585
• Protein ID: WP_001216039.1
• Coordinates: 40051..40431 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: LPH32_RS23580
• Protein ID: WP_001190711.1
• Coordinates: 39830..40051 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH32_RS23550 (VEGS19_44970)	35026..35454	+	429	Protein_40	metallophosphoesterase	-
LPH32_RS23555 (VEGS19_44980)	35518..36222	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LPH32_RS23560	36274..36888	+	615	Protein_42	hypothetical protein	-
LPH32_RS23565 (VEGS19_45010)	37190..38452	+	1263	WP_069067570.1	hypothetical protein	-
LPH32_RS23570 (VEGS19_45020)	38525..38851	+	327	Protein_44	hypothetical protein	-
LPH32_RS23580 (VEGS19_45050)	39830..40051	+	222	WP_001190711.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LPH32_RS23585 (VEGS19_45060)	40051..40431	+	381	WP_001216039.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LPH32_RS23590 (VEGS19_45070)	40436..40615	+	180	WP_000113018.1	hypothetical protein	-
LPH32_RS23595 (VEGS19_45080)	40643..41686	+	1044	WP_069067567.1	DUF968 domain-containing protein	-
LPH32_RS23600 (VEGS19_45100)	41902..42300	+	399	WP_229436862.1	phage antirepressor KilAC domain-containing protein	-
LPH32_RS23605 (VEGS19_45110)	42333..43184	-	852	WP_000611661.1	phage repressor protein C1	-
LPH32_RS23610 (VEGS19_45120)	43295..43504	-	210	WP_000874156.1	hypothetical protein	-
LPH32_RS23615 (VEGS19_45130)	44109..44330	+	222	WP_000542338.1	hypothetical protein	-
LPH32_RS23620 (VEGS19_45140)	44338..45369	+	1032	WP_000067537.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / qnrS1 / dfrA14 / blaTEM-1C / sul2 / aph(3'')-Ib / aph(6)-Id / floR	-	1..83929	83929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13558.27 Da
• Isoelectric Point: 5.1514

>T40143 WP_001216039.1 NZ_AP025216:40051-40431 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRAALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T40143 NZ_AP025216:40051-40431 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTGCCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8134.08 Da
• Isoelectric Point: 4.5843

>AT40143 WP_001190711.1 NZ_AP025216:39830-40051 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVDR

Nucleotide

• Length: 222 bp

>AT40143 NZ_AP025216:39830-40051 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCTCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTGACCGATAA