Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 6470..7005 Replicon plasmid p20170902CC-2
Accession NZ_AP025216
Organism Escherichia coli strain 2017.09.02CC

Toxin (Protein)


Gene name relE Uniprot ID A0A0K4CX11
Locus tag LPH32_RS23380 Protein ID WP_000222771.1
Coordinates 6470..6757 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T1PXV2
Locus tag LPH32_RS23385 Protein ID WP_001139206.1
Coordinates 6754..7005 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH32_RS23365 (VEGS19_44560) 2095..5610 + 3516 WP_077491875.1 autotransporter outer membrane beta-barrel domain-containing protein -
LPH32_RS23370 5785..5990 - 206 Protein_4 hypothetical protein -
LPH32_RS23375 5989..6102 + 114 Protein_5 alkaline phosphatase -
LPH32_RS23380 (VEGS19_44580) 6470..6757 - 288 WP_000222771.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LPH32_RS23385 (VEGS19_44590) 6754..7005 - 252 WP_001139206.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LPH32_RS23390 (VEGS19_44600) 7688..8119 - 432 Protein_8 IncHI-type conjugal transfer protein TrhH -
LPH32_RS23395 (VEGS19_44610) 8109..8531 - 423 WP_001352023.1 conjugal transfer protein TraF -
LPH32_RS23400 (VEGS19_44620) 8482..9156 - 675 WP_001352022.1 conjugal transfer protein TraF -
LPH32_RS23405 (VEGS19_44630) 9943..10281 - 339 WP_223656872.1 hypothetical protein -
LPH32_RS23410 (VEGS19_44640) 10284..10814 - 531 WP_229436842.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / qnrS1 / dfrA14 / blaTEM-1C / sul2 / aph(3'')-Ib / aph(6)-Id / floR - 1..83929 83929


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11116.17 Da        Isoelectric Point: 10.5833

>T40142 WP_000222771.1 NZ_AP025216:c6757-6470 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYHLASERMK

Download         Length: 288 bp

>T40142 NZ_AP025216:c6757-6470 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9171.39 Da        Isoelectric Point: 4.0927

>AT40142 WP_001139206.1 NZ_AP025216:c7005-6754 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Download         Length: 252 bp

>AT40142 NZ_AP025216:c7005-6754 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4CX11

Antitoxin

Source ID Structure
AlphaFold DB T1PXV2

References