Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 35126..35924 Replicon plasmid p20170902CC-1
Accession NZ_AP025215
Organism Escherichia coli strain 2017.09.02CC

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag LPH32_RS22920 Protein ID WP_000072677.1
Coordinates 35403..35924 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag LPH32_RS22915 Protein ID WP_001351987.1
Coordinates 35126..35395 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH32_RS22895 (VEGS19_A43660) 31115..31855 - 741 WP_001755535.1 hypothetical protein -
LPH32_RS22900 (VEGS19_A43670) 32034..33728 - 1695 WP_000443723.1 hypothetical protein -
LPH32_RS22905 (VEGS19_A43680) 33780..34133 - 354 WP_160378290.1 hypothetical protein -
LPH32_RS22910 (VEGS19_A43690) 34139..34807 - 669 WP_000161228.1 division plane positioning ATPase MipZ -
LPH32_RS22915 (VEGS19_A43700) 35126..35395 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
LPH32_RS22920 (VEGS19_A43710) 35403..35924 + 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
LPH32_RS22925 (VEGS19_A43720) 36093..36344 - 252 WP_000901559.1 hypothetical protein -
LPH32_RS22930 (VEGS19_A43730) 36346..37038 - 693 WP_012640731.1 hypothetical protein -
LPH32_RS22935 (VEGS19_A43740) 37052..37375 - 324 WP_000064175.1 hypothetical protein -
LPH32_RS22940 (VEGS19_A43750) 37579..40242 - 2664 WP_229436825.1 phage tail protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..110874 110874


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T40141 WP_000072677.1 NZ_AP025215:35403-35924 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T40141 NZ_AP025215:35403-35924 [Escherichia coli]
GTGAGCAATACAACGATAGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCCTTTTTAGCCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGTGCTTATGTGCTTTGCACTCAAGAAG
AAAGGCCAAAAGTGTTAGGATATTACACTTTGTCAGGTAGTTGTTTTGAAAAGGAGTCCTTACCTTCAAGAAGCAAACAA
AAGAAGGTTCCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGCATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT40141 WP_001351987.1 NZ_AP025215:35126-35395 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp

>AT40141 NZ_AP025215:35126-35395 [Escherichia coli]
ATGTCAGCACTAAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACTCAGTTTATGGTTAGCAGTGCATCTGAACGTGCCGTGAAAGTTATAGAGCAACACCGTAGAC
TAGTTCTGAATGAAGAATCCTGGAATCTGGTTATGGACGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCTGATCGTCTGAAAAGCATGGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References