Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4288996..4289621 Replicon chromosome
Accession NZ_AP025214
Organism Escherichia coli strain 2017.09.02CC

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag LPH32_RS20820 Protein ID WP_000911329.1
Coordinates 4289223..4289621 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag LPH32_RS20815 Protein ID WP_000450524.1
Coordinates 4288996..4289223 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH32_RS20790 (4284796) 4284796..4285266 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
LPH32_RS20795 (4285266) 4285266..4285838 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
LPH32_RS20800 (4285984) 4285984..4286862 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
LPH32_RS20805 (4286879) 4286879..4287913 + 1035 WP_001296286.1 outer membrane protein assembly factor BamC -
LPH32_RS20810 (4288126) 4288126..4288839 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
LPH32_RS20815 (4288996) 4288996..4289223 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
LPH32_RS20820 (4289223) 4289223..4289621 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
LPH32_RS20825 (4289768) 4289768..4290631 + 864 WP_001267498.1 neutral zinc metallopeptidase -
LPH32_RS20830 (4290646) 4290646..4292661 + 2016 WP_053291038.1 tRNA cytosine(34) acetyltransferase TmcA -
LPH32_RS20835 (4292735) 4292735..4293433 + 699 WP_001578773.1 esterase -
LPH32_RS20840 (4293514) 4293514..4293714 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T40140 WP_000911329.1 NZ_AP025214:4289223-4289621 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T40140 NZ_AP025214:4289223-4289621 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT40140 WP_000450524.1 NZ_AP025214:4288996-4289223 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT40140 NZ_AP025214:4288996-4289223 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References