Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3854744..3855398 Replicon chromosome
Accession NZ_AP025214
Organism Escherichia coli strain 2017.09.02CC

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LPH32_RS18860 Protein ID WP_000244781.1
Coordinates 3854991..3855398 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LPH32_RS18855 Protein ID WP_000354046.1
Coordinates 3854744..3855010 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH32_RS18835 (3850822) 3850822..3852255 - 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -
LPH32_RS18840 (3852300) 3852300..3852611 + 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
LPH32_RS18845 (3852775) 3852775..3853434 + 660 WP_000250269.1 hemolysin III family protein -
LPH32_RS18850 (3853511) 3853511..3854491 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
LPH32_RS18855 (3854744) 3854744..3855010 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LPH32_RS18860 (3854991) 3854991..3855398 + 408 WP_000244781.1 protein YgfX Toxin
LPH32_RS18865 (3855438) 3855438..3855959 - 522 WP_001055874.1 flavodoxin FldB -
LPH32_RS18870 (3856071) 3856071..3856967 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LPH32_RS18875 (3856992) 3856992..3857702 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LPH32_RS18880 (3857708) 3857708..3859441 + 1734 WP_053290563.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T40139 WP_000244781.1 NZ_AP025214:3854991-3855398 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T40139 NZ_AP025214:3854991-3855398 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT40139 WP_000354046.1 NZ_AP025214:3854744-3855010 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT40139 NZ_AP025214:3854744-3855010 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References