Detailed information of TA system TA040137

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3284802..3285397
• Replicon: chromosome
• Accession: NZ_AP025214
• Organism: Escherichia coli strain 2017.09.02CC

Toxin (Protein)

• Gene name: doc
• Uniprot ID: D6JG31
• Locus tag: LPH32_RS16000
• Protein ID: WP_000155159.1
• Coordinates: 3285020..3285397 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D6JG32
• Locus tag: LPH32_RS15995
• Protein ID: WP_000557315.1
• Coordinates: 3284802..3285023 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH32_RS15970 (3279878)	3279878..3280987	+	1110	WP_001316697.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LPH32_RS15975 (3281035)	3281035..3281961	+	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LPH32_RS15980 (3281958)	3281958..3283235	+	1278	WP_053290830.1	branched chain amino acid ABC transporter permease LivM	-
LPH32_RS15985 (3283232)	3283232..3283999	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LPH32_RS15990 (3284001)	3284001..3284714	+	714	WP_000416895.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LPH32_RS15995 (3284802)	3284802..3285023	+	222	WP_000557315.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LPH32_RS16000 (3285020)	3285020..3285397	+	378	WP_000155159.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LPH32_RS16005 (3285606)	3285606..3286922	+	1317	WP_000803191.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LPH32_RS16010 (3287098)	3287098..3287985	+	888	WP_000099289.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LPH32_RS16015 (3287982)	3287982..3288827	+	846	WP_000572164.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LPH32_RS16020 (3288829)	3288829..3289899	+	1071	WP_000907828.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3281958..3290639	8681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (27-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13964.25 Da
• Isoelectric Point: 6.7263

>T40137 WP_000155159.1 NZ_AP025214:3285020-3285397 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILAANPEFVELTVDVAAGKVSLEDIVTRLRQRGT

Nucleotide

• Length: 378 bp

>T40137 NZ_AP025214:3285020-3285397 [Escherichia coli]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGCGTTTCTGATCTTT
GGTTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTAGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACGCGGTACCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8103.24 Da
• Isoelectric Point: 6.2168

>AT40137 WP_000557315.1 NZ_AP025214:3284802-3285023 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIKELADK

Nucleotide

• Length: 222 bp

>AT40137 NZ_AP025214:3284802-3285023 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTAGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCAGCTGTGATTATCAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CAGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: D6JG31

Antitoxin

• AlphaFold DB: D6JG32