Detailed information of TA system TA040136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3257526..3258157
• Replicon: chromosome
• Accession: NZ_AP025214
• Organism: Escherichia coli strain 2017.09.02CC

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: LPH32_RS15845
• Protein ID: WP_001260301.1
• Coordinates: 3257526..3257801 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: LPH32_RS15850
• Protein ID: WP_000593557.1
• Coordinates: 3257798..3258157 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH32_RS15830 (3252530)	3252530..3253597	+	1068	WP_000361470.1	HlyD family secretion protein	-
LPH32_RS15835 (3253594)	3253594..3256329	+	2736	WP_000149178.1	ribosome-associated ATPase/putative transporter RbbA	-
LPH32_RS15840 (3256329)	3256329..3257453	+	1125	WP_001318088.1	ABC transporter permease	-
LPH32_RS15845 (3257526)	3257526..3257801	+	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
LPH32_RS15850 (3257798)	3257798..3258157	+	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LPH32_RS15855 (3258277)	3258277..3258678	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
LPH32_RS15860 (3258684)	3258684..3259490	-	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
LPH32_RS15865 (3259487)	3259487..3260251	-	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
LPH32_RS15870 (3260251)	3260251..3261084	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
LPH32_RS15875 (3261081)	3261081..3262025	-	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T40136 WP_001260301.1 NZ_AP025214:3257526-3257801 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T40136 NZ_AP025214:3257526-3257801 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTT
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT40136 WP_000593557.1 NZ_AP025214:3257798-3258157 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT40136 NZ_AP025214:3257798-3258157 [Escherichia coli]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1