Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2486195..2486692 Replicon chromosome
Accession NZ_AP025214
Organism Escherichia coli strain 2017.09.02CC

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LPH32_RS12310 Protein ID WP_001173343.1
Coordinates 2486195..2486368 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A1V2FLJ5
Locus tag LPH32_RS12315 Protein ID WP_001317561.1
Coordinates 2486396..2486692 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH32_RS12300 (2482865) 2482865..2484322 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
LPH32_RS12305 (2484559) 2484559..2486076 + 1518 WP_229436148.1 lysine--tRNA ligase -
LPH32_RS12310 (2486195) 2486195..2486368 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LPH32_RS12315 (2486396) 2486396..2486692 - 297 WP_001317561.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LPH32_RS12320 (2486919) 2486919..2487191 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LPH32_RS12325 (2487203) 2487203..2487433 - 231 WP_000371717.1 4Fe-4S mono-cluster protein YjdI -
LPH32_RS12330 (2487613) 2487613..2489244 + 1632 WP_001216477.1 sensor histidine kinase -
LPH32_RS12335 (2489241) 2489241..2489960 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T40132 WP_001173343.1 NZ_AP025214:c2486368-2486195 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T40132 NZ_AP025214:c2486368-2486195 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT40132 WP_001317561.1 NZ_AP025214:c2486692-2486396 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT40132 NZ_AP025214:c2486692-2486396 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAGTTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2FLJ5

References