Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1698006..1698624 Replicon chromosome
Accession NZ_AP025214
Organism Escherichia coli strain 2017.09.02CC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LPH32_RS08545 Protein ID WP_001291435.1
Coordinates 1698406..1698624 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LPH32_RS08540 Protein ID WP_000344800.1
Coordinates 1698006..1698380 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH32_RS08530 (1693095) 1693095..1694288 + 1194 WP_053290608.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LPH32_RS08535 (1694311) 1694311..1697460 + 3150 WP_229436609.1 efflux RND transporter permease AcrB -
LPH32_RS08540 (1698006) 1698006..1698380 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LPH32_RS08545 (1698406) 1698406..1698624 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LPH32_RS08550 (1698796) 1698796..1699347 + 552 WP_000102564.1 maltose O-acetyltransferase -
LPH32_RS08555 (1699463) 1699463..1699933 + 471 WP_000136192.1 YlaC family protein -
LPH32_RS08560 (1700097) 1700097..1701647 + 1551 WP_001317659.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LPH32_RS08565 (1701689) 1701689..1702042 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LPH32_RS08575 (1702421) 1702421..1702732 + 312 WP_000409911.1 MGMT family protein -
LPH32_RS08580 (1702763) 1702763..1703335 - 573 WP_000779821.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T40130 WP_001291435.1 NZ_AP025214:1698406-1698624 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T40130 NZ_AP025214:1698406-1698624 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT40130 WP_000344800.1 NZ_AP025214:1698006-1698380 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT40130 NZ_AP025214:1698006-1698380 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References