Detailed information of TA system TA040128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1291668..1292373
• Replicon: chromosome
• Accession: NZ_AP025214
• Organism: Escherichia coli strain 2017.09.02CC

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: LPH32_RS06485
• Protein ID: WP_000539521.1
• Coordinates: 1291668..1292054 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LPH32_RS06490
• Protein ID: WP_001280945.1
• Coordinates: 1292044..1292373 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH32_RS06465 (1287672)	1287672..1288298	+	627	WP_001295292.1	glutathione S-transferase GstB	-
LPH32_RS06470 (1288295)	1288295..1289410	-	1116	WP_000555041.1	aldose sugar dehydrogenase YliI	-
LPH32_RS06475 (1289521)	1289521..1289904	-	384	WP_053290883.1	biofilm formation regulator BssR	-
LPH32_RS06480 (1290117)	1290117..1291442	+	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
LPH32_RS06485 (1291668)	1291668..1292054	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LPH32_RS06490 (1292044)	1292044..1292373	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
LPH32_RS06495 (1292443)	1292443..1293771	-	1329	WP_000086919.1	GGDEF domain-containing protein	-
LPH32_RS06500 (1293779)	1293779..1296136	-	2358	WP_229436497.1	EAL domain-containing protein	-
LPH32_RS06505 (1296314)	1296314..1297225	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T40128 WP_000539521.1 NZ_AP025214:1291668-1292054 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T40128 NZ_AP025214:1291668-1292054 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT40128 WP_001280945.1 NZ_AP025214:1292044-1292373 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT40128 NZ_AP025214:1292044-1292373 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6