Detailed information of TA system TA040126

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 715318..715954
• Replicon: chromosome
• Accession: NZ_AP025214
• Organism: Escherichia coli strain 2017.09.02CC

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: LPH32_RS03635
• Protein ID: WP_000813796.1
• Coordinates: 715778..715954 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LPH32_RS03630
• Protein ID: WP_076838470.1
• Coordinates: 715318..715734 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH32_RS03610 (710470)	710470..711411	-	942	WP_053290975.1	ABC transporter permease	-
LPH32_RS03615 (711412)	711412..712425	-	1014	WP_053290974.1	ABC transporter ATP-binding protein	-
LPH32_RS03620 (712443)	712443..713588	-	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
LPH32_RS03625 (713833)	713833..715239	-	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
LPH32_RS03630 (715318)	715318..715734	-	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LPH32_RS03635 (715778)	715778..715954	-	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LPH32_RS03640 (716176)	716176..716406	+	231	WP_024196315.1	DUF2554 family protein	-
LPH32_RS03645 (716498)	716498..718459	-	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LPH32_RS03650 (718532)	718532..719068	-	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
LPH32_RS03655 (719160)	719160..720332	+	1173	WP_001236216.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T40126 WP_000813796.1 NZ_AP025214:c715954-715778 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T40126 NZ_AP025214:c715954-715778 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT40126 WP_076838470.1 NZ_AP025214:c715734-715318 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT40126 NZ_AP025214:c715734-715318 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACTCCTGCGCCGGAAGGCGGTTATGTGGTTTCTTTTGTAGATATCCCTGAAGCGTTGAC
GCAGGGAGAAACTGTTGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCTACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3