Detailed information of TA system TA040115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 165628..166229
• Replicon: plasmid p20170604CC-1
• Accession: NZ_AP025210
• Organism: Escherichia coli strain 2017.06.04CC

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: LPH28_RS23260
• Protein ID: WP_088213501.1
• Coordinates: 165628..166008 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: LPH28_RS23265
• Protein ID: WP_001190712.1
• Coordinates: 166008..166229 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH28_RS23225 (VEGS18_A45060)	160753..160974	-	222	WP_000542336.1	hypothetical protein	-
LPH28_RS23230 (VEGS18_A45070)	161579..161788	+	210	WP_063501801.1	hypothetical protein	-
LPH28_RS23235 (VEGS18_A45080)	161899..162750	+	852	WP_000611656.1	phage repressor protein C1	-
LPH28_RS23240	162783..163901	-	1119	Protein_172	phage antirepressor KilAC domain-containing protein	-
LPH28_RS23245 (VEGS18_A45110)	163898..164119	-	222	WP_001361625.1	host cell division inhibitor Icd-like protein	-
LPH28_RS23250 (VEGS18_A45120)	164373..165416	-	1044	WP_088213502.1	DUF968 domain-containing protein	-
LPH28_RS23255	165444..165623	-	180	WP_001411052.1	hypothetical protein	-
LPH28_RS23260 (VEGS18_A45130)	165628..166008	-	381	WP_088213501.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LPH28_RS23265 (VEGS18_A45140)	166008..166229	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LPH28_RS23270 (VEGS18_A45150)	166412..167968	+	1557	WP_088213504.1	type I restriction-modification system subunit M	-
LPH28_RS23275 (VEGS18_A45160)	167965..169149	+	1185	WP_072796199.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	dfrA1 / ant(3'')-Ia / qacE / sul1 / mph(A) / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / floR / sul2 / aadA2 / dfrA12 / qnrS1 / tet(A)	-	1..216026	216026

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13642.43 Da
• Isoelectric Point: 5.1473

>T40115 WP_088213501.1 NZ_AP025210:c166008-165628 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGYIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGTATGEISVSTVAATLRRLYGSAE

Nucleotide

• Length: 381 bp

>T40115 NZ_AP025210:c166008-165628 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGTATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGACTGCGACTGG
CGAGATATCTGTATCTACTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT40115 WP_001190712.1 NZ_AP025210:c166229-166008 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT40115 NZ_AP025210:c166229-166008 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6