40114

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 80379..80914 Replicon plasmid p20170604CC-1
Accession NZ_AP025210
Organism Escherichia coli strain 2017.06.04CC

Toxin (Protein)


Gene name relE Uniprot ID A0A0K4CX11
Locus tag LPH28_RS22750 Protein ID WP_000222771.1
Coordinates 80379..80666 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T1PXV2
Locus tag LPH28_RS22755 Protein ID WP_001139206.1
Coordinates 80663..80914 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH28_RS22735 (VEGS18_A44010) 76004..79519 + 3516 WP_072664206.1 autotransporter outer membrane beta-barrel domain-containing protein -
LPH28_RS22740 79694..79899 - 206 Protein_72 hypothetical protein -
LPH28_RS22745 79898..80011 + 114 Protein_73 alkaline phosphatase -
LPH28_RS22750 (VEGS18_A44030) 80379..80666 - 288 WP_000222771.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LPH28_RS22755 (VEGS18_A44040) 80663..80914 - 252 WP_001139206.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LPH28_RS22760 (VEGS18_A44050) 81497..81766 + 270 Protein_76 hypothetical protein -
LPH28_RS22765 (VEGS18_A44060) 81995..83395 + 1401 WP_032185977.1 cytochrome ubiquinol oxidase subunit I -
LPH28_RS22770 (VEGS18_A44070) 83395..84405 + 1011 WP_000510495.1 cytochrome d ubiquinol oxidase subunit II -
LPH28_RS22775 84405..84533 + 129 WP_001164804.1 DUF2474 domain-containing protein -
LPH28_RS22780 (VEGS18_A44080) 84638..85051 - 414 Protein_80 collagenase-like protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid dfrA1 / ant(3'')-Ia / qacE / sul1 / mph(A) / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / floR / sul2 / aadA2 / dfrA12 / qnrS1 / tet(A) - 1..216026 216026


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11116.17 Da        Isoelectric Point: 10.5833

>T40114 WP_000222771.1 NZ_AP025210:c80666-80379 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYHLASERMK

Download         Length: 288 bp

>T40114 NZ_AP025210:c80666-80379 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9171.39 Da        Isoelectric Point: 4.0927

>AT40114 WP_001139206.1 NZ_AP025210:c80914-80663 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Download         Length: 252 bp

>AT40114 NZ_AP025210:c80914-80663 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4CX11

Antitoxin

Source ID Structure
AlphaFold DB T1PXV2

References