40107

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2385703..2386341 Replicon chromosome
Accession NZ_AP025209
Organism Escherichia coli strain 2017.06.04CC

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag LPH28_RS11470 Protein ID WP_000813794.1
Coordinates 2386165..2386341 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LPH28_RS11465 Protein ID WP_001270286.1
Coordinates 2385703..2386119 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH28_RS11445 (2380855) 2380855..2381796 - 942 WP_001251319.1 ABC transporter permease -
LPH28_RS11450 (2381797) 2381797..2382810 - 1014 WP_000220393.1 ABC transporter ATP-binding protein -
LPH28_RS11455 (2382828) 2382828..2383973 - 1146 WP_112951933.1 ABC transporter substrate-binding protein -
LPH28_RS11460 (2384215) 2384215..2385624 - 1410 WP_000760625.1 PLP-dependent aminotransferase family protein -
LPH28_RS11465 (2385703) 2385703..2386119 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LPH28_RS11470 (2386165) 2386165..2386341 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LPH28_RS11475 (2386563) 2386563..2386793 + 231 WP_112951934.1 YncJ family protein -
LPH28_RS11480 (2386885) 2386885..2388846 - 1962 WP_229434161.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LPH28_RS11485 (2388919) 2388919..2389455 - 537 WP_112951935.1 DNA-binding transcriptional regulator SutR -
LPH28_RS11490 (2389547) 2389547..2390722 + 1176 WP_001236265.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T40107 WP_000813794.1 NZ_AP025209:c2386341-2386165 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T40107 NZ_AP025209:c2386341-2386165 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT40107 WP_001270286.1 NZ_AP025209:c2386119-2385703 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT40107 NZ_AP025209:c2386119-2385703 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References