Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 64805..65340 Replicon plasmid p20170403CC-1
Accession NZ_AP025206
Organism Escherichia coli strain 2017.04.03CC

Toxin (Protein)


Gene name relE Uniprot ID A0A0K4CX11
Locus tag LPH27_RS22245 Protein ID WP_000222771.1
Coordinates 65053..65340 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T1PXV2
Locus tag LPH27_RS22240 Protein ID WP_001139206.1
Coordinates 64805..65056 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH27_RS22215 (VEGS17_A43190) 60668..61081 + 414 Protein_68 collagenase-like protease -
LPH27_RS22220 61186..61314 - 129 WP_001164804.1 DUF2474 domain-containing protein -
LPH27_RS22225 (VEGS17_A43200) 61314..62324 - 1011 WP_000510495.1 cytochrome d ubiquinol oxidase subunit II -
LPH27_RS22230 (VEGS17_A43210) 62324..63724 - 1401 WP_032185977.1 cytochrome ubiquinol oxidase subunit I -
LPH27_RS22235 (VEGS17_A43220) 63953..64222 - 270 Protein_72 hypothetical protein -
LPH27_RS22240 (VEGS17_A43230) 64805..65056 + 252 WP_001139206.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LPH27_RS22245 (VEGS17_A43240) 65053..65340 + 288 WP_000222771.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LPH27_RS22250 65708..65821 - 114 Protein_75 alkaline phosphatase -
LPH27_RS22255 (VEGS17_A43260) 65820..66025 + 206 Protein_76 hypothetical protein -
LPH27_RS22260 (VEGS17_A43270) 66200..69697 - 3498 WP_088209975.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / qnrS1 / floR / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..92278 92278


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11116.17 Da        Isoelectric Point: 10.5833

>T40090 WP_000222771.1 NZ_AP025206:65053-65340 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYHLASERMK

Download         Length: 288 bp

>T40090 NZ_AP025206:65053-65340 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9171.39 Da        Isoelectric Point: 4.0927

>AT40090 WP_001139206.1 NZ_AP025206:64805-65056 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Download         Length: 252 bp

>AT40090 NZ_AP025206:64805-65056 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAAGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4CX11

Antitoxin

Source ID Structure
AlphaFold DB T1PXV2

References