Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 6706..7307 Replicon plasmid p20170403CC-1
Accession NZ_AP025206
Organism Escherichia coli strain 2017.04.03CC

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag LPH27_RS21915 Protein ID WP_001216034.1
Coordinates 6927..7307 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag LPH27_RS21910 Protein ID WP_001190712.1
Coordinates 6706..6927 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH27_RS21880 (VEGS17_A42440) 1815..2321 + 507 WP_000021754.1 hypothetical protein -
LPH27_RS21885 (VEGS17_A42450) 2516..3694 + 1179 WP_094318253.1 hypothetical protein -
LPH27_RS21890 (VEGS17_A42460) 3822..4052 + 231 WP_223656858.1 hypothetical protein -
LPH27_RS21895 (VEGS17_A42470) 5128..5484 - 357 WP_001062545.1 hypothetical protein -
LPH27_RS21900 (VEGS17_A42480) 5485..5892 - 408 WP_223656859.1 hypothetical protein -
LPH27_RS21905 (VEGS17_A42490) 6244..6633 + 390 WP_000506724.1 S24 family peptidase -
LPH27_RS21910 (VEGS17_A42500) 6706..6927 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LPH27_RS21915 (VEGS17_A42510) 6927..7307 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LPH27_RS21920 (VEGS17_A42520) 7312..7491 + 180 WP_000113018.1 hypothetical protein -
LPH27_RS21925 (VEGS17_A42530) 7519..8562 + 1044 WP_112918711.1 DUF968 domain-containing protein -
LPH27_RS21930 (VEGS17_A42540) 8651..9103 + 453 WP_001326849.1 late promoter-activating protein -
LPH27_RS21935 (VEGS17_A42550) 9189..10382 + 1194 WP_032214401.1 hypothetical protein -
LPH27_RS21940 (VEGS17_A42560) 10382..11866 + 1485 WP_000124159.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / qnrS1 / floR / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..92278 92278


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T40089 WP_001216034.1 NZ_AP025206:6927-7307 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T40089 NZ_AP025206:6927-7307 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTCGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT40089 WP_001190712.1 NZ_AP025206:6706-6927 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT40089 NZ_AP025206:6706-6927 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References