Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3889098..3889674 Replicon chromosome
Accession NZ_AP025205
Organism Escherichia coli strain 2017.04.03CC

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LPH27_RS18710 Protein ID WP_001297643.1
Coordinates 3889387..3889674 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag LPH27_RS18705 Protein ID WP_000063156.1
Coordinates 3889098..3889400 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH27_RS18690 (3885694) 3885694..3887844 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LPH27_RS18695 (3887894) 3887894..3888097 + 204 WP_000467859.1 YbdD/YjiX family protein -
LPH27_RS18700 (3888108) 3888108..3889064 + 957 WP_001297640.1 GTPase -
LPH27_RS18705 (3889098) 3889098..3889400 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
LPH27_RS18710 (3889387) 3889387..3889674 - 288 WP_001297643.1 BrnT family toxin Toxin
LPH27_RS18715 (3889873) 3889873..3890570 - 698 Protein_3655 IS1 family transposase -
LPH27_RS18720 (3890627) 3890627..3890722 + 96 Protein_3656 dienelactone hydrolase family protein -
LPH27_RS18725 (3890868) 3890868..3892100 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
LPH27_RS18730 (3892141) 3892141..3893421 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3889873..3890082 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T40086 WP_001297643.1 NZ_AP025205:c3889674-3889387 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T40086 NZ_AP025205:c3889674-3889387 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT40086 WP_000063156.1 NZ_AP025205:c3889400-3889098 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT40086 NZ_AP025205:c3889400-3889098 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References