Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3372359..3372977 Replicon chromosome
Accession NZ_AP025205
Organism Escherichia coli strain 2017.04.03CC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LPH27_RS16210 Protein ID WP_001291435.1
Coordinates 3372759..3372977 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LPH27_RS16205 Protein ID WP_000344800.1
Coordinates 3372359..3372733 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH27_RS16195 (3367448) 3367448..3368641 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LPH27_RS16200 (3368664) 3368664..3371813 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LPH27_RS16205 (3372359) 3372359..3372733 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LPH27_RS16210 (3372759) 3372759..3372977 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LPH27_RS16220 (3373926) 3373926..3374477 + 552 WP_000102564.1 maltose O-acetyltransferase -
LPH27_RS16225 (3374593) 3374593..3375063 + 471 WP_000136192.1 YlaC family protein -
LPH27_RS16230 (3375227) 3375227..3376777 + 1551 WP_229266155.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LPH27_RS16235 (3376819) 3376819..3377172 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LPH27_RS16245 (3377551) 3377551..3377862 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T40083 WP_001291435.1 NZ_AP025205:3372759-3372977 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T40083 NZ_AP025205:3372759-3372977 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT40083 WP_000344800.1 NZ_AP025205:3372359-3372733 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT40083 NZ_AP025205:3372359-3372733 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References