Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/YoeB-YefM
Location 5323933..5324432 Replicon chromosome
Accession NZ_AP025186
Organism Rhodococcus sp. RDE2

Toxin (Protein)


Gene name relE Uniprot ID V9XF56
Locus tag LPH33_RS24885 Protein ID WP_024102500.1
Coordinates 5323933..5324187 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0V9UIF5
Locus tag LPH33_RS24890 Protein ID WP_060652490.1
Coordinates 5324184..5324432 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH33_RS24855 (RDE2_49650) 5319365..5320534 - 1170 WP_229566549.1 acyl-CoA dehydrogenase family protein -
LPH33_RS24860 (RDE2_49660) 5320637..5321263 + 627 WP_006550849.1 TetR family transcriptional regulator -
LPH33_RS24865 (RDE2_49670) 5321421..5321633 - 213 WP_060652491.1 hypothetical protein -
LPH33_RS24870 (RDE2_49680) 5321640..5321873 - 234 WP_267499200.1 transposase -
LPH33_RS24875 (RDE2_49690) 5322198..5323154 - 957 WP_019290518.1 ERCC4 domain-containing protein -
LPH33_RS24880 (RDE2_49700) 5323311..5323832 + 522 WP_229566551.1 hypothetical protein -
LPH33_RS24885 (RDE2_49710) 5323933..5324187 - 255 WP_024102500.1 Txe/YoeB family addiction module toxin Toxin
LPH33_RS24890 (RDE2_49720) 5324184..5324432 - 249 WP_060652490.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
LPH33_RS26865 (RDE2_49730) 5324528..5324797 + 270 WP_267499182.1 pirin-like C-terminal cupin domain-containing protein -
LPH33_RS24900 (RDE2_49740) 5325266..5326060 + 795 WP_229566552.1 LLM class F420-dependent oxidoreductase -
LPH33_RS24905 (RDE2_49750) 5326121..5327353 + 1233 WP_229566553.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-70)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10077.42 Da        Isoelectric Point: 8.0410

>T40061 WP_024102500.1 NZ_AP025186:c5324187-5323933 [Rhodococcus sp. RDE2]
VNITFTDEAWDDYQFWISTDRKQLKRVNELIRDCQRDPYSGIGKPERLRHALSGLWSRRIDKEHRLVYEVIDGALVIIAA
RYHY

Download         Length: 255 bp

>T40061 NZ_AP025186:c5324187-5323933 [Rhodococcus sp. RDE2]
GTGAACATCACCTTCACCGACGAAGCCTGGGACGACTACCAGTTCTGGATCTCGACCGACAGAAAACAGCTCAAGCGTGT
CAACGAGCTGATCAGGGATTGCCAACGAGACCCGTACTCGGGGATCGGCAAACCCGAACGGCTCAGACATGCCCTGTCAG
GACTGTGGTCTCGACGTATCGACAAAGAACACCGCCTGGTCTACGAGGTCATCGACGGTGCCCTGGTCATCATCGCCGCC
CGCTACCACTACTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9276.56 Da        Isoelectric Point: 5.8315

>AT40061 WP_060652490.1 NZ_AP025186:c5324432-5324184 [Rhodococcus sp. RDE2]
MAAITATEARKNFFPLVEQVNKDHTPVEIVSKRGNAVLMSKDDYDAMLETSYLMQSPANARRLLASMQRARGGEYTEREL
IE

Download         Length: 249 bp

>AT40061 NZ_AP025186:c5324432-5324184 [Rhodococcus sp. RDE2]
ATGGCTGCCATCACTGCTACCGAAGCGAGAAAAAACTTCTTTCCGCTGGTCGAACAGGTCAACAAGGACCACACCCCTGT
GGAAATCGTATCCAAGCGGGGCAATGCCGTCCTGATGTCGAAGGATGACTACGACGCCATGCTCGAGACCTCGTACCTGA
TGCAGTCTCCGGCCAACGCCCGACGACTGCTCGCCAGCATGCAGAGAGCTCGCGGAGGTGAATACACCGAAAGGGAACTC
ATCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7M2XPP3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9UIF5

References