Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4071474..4072092 Replicon chromosome
Accession NZ_AP025180
Organism Escherichia coli strain JP538

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag R5G_RS19980 Protein ID WP_001291435.1
Coordinates 4071474..4071692 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag R5G_RS19985 Protein ID WP_000344800.1
Coordinates 4071718..4072092 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R5G_RS19945 (4066763) 4066763..4067335 + 573 WP_000779831.1 YbaY family lipoprotein -
R5G_RS19950 (4067366) 4067366..4067677 - 312 WP_000409911.1 MGMT family protein -
R5G_RS19960 (4068056) 4068056..4068409 + 354 WP_000878140.1 DUF1428 domain-containing protein -
R5G_RS19965 (4068451) 4068451..4070001 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
R5G_RS19970 (4070165) 4070165..4070635 - 471 WP_000136192.1 YlaC family protein -
R5G_RS19975 (4070751) 4070751..4071302 - 552 WP_000102564.1 maltose O-acetyltransferase -
R5G_RS19980 (4071474) 4071474..4071692 - 219 WP_001291435.1 HHA domain-containing protein Toxin
R5G_RS19985 (4071718) 4071718..4072092 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
R5G_RS19990 (4072638) 4072638..4075787 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
R5G_RS19995 (4075810) 4075810..4077003 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T40051 WP_001291435.1 NZ_AP025180:c4071692-4071474 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T40051 NZ_AP025180:c4071692-4071474 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT40051 WP_000344800.1 NZ_AP025180:c4072092-4071718 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT40051 NZ_AP025180:c4072092-4071718 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References