Detailed information of TA system TA040025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 6117008..6117687
• Replicon: chromosome
• Accession: NZ_AP025179
• Organism: Mycobacterium kiyosense strain IWGMT90018-18076

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L6444_RS28960
• Protein ID: WP_236978924.1
• Coordinates: 6117283..6117687 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L6444_RS28955
• Protein ID: WP_236978923.1
• Coordinates: 6117008..6117286 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6444_RS28935 (IWGMT90018_60440)	6112798..6113706	-	909	WP_255731129.1	CoA ester lyase	-
L6444_RS28940 (IWGMT90018_60450)	6113720..6114904	-	1185	WP_236978917.1	CoA transferase	-
L6444_RS28945 (IWGMT90018_60460)	6114897..6116243	-	1347	WP_236978919.1	LLM class flavin-dependent oxidoreductase	-
L6444_RS28950 (IWGMT90018_60470)	6116313..6116957	+	645	WP_236978921.1	GntR family transcriptional regulator	-
L6444_RS28955 (IWGMT90018_60480)	6117008..6117286	+	279	WP_236978923.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
L6444_RS28960 (IWGMT90018_60490)	6117283..6117687	+	405	WP_236978924.1	type II toxin-antitoxin system VapC family toxin	Toxin
L6444_RS28965 (IWGMT90018_60500)	6118088..6118984	+	897	WP_236978926.1	helix-turn-helix transcriptional regulator	-
L6444_RS28970 (IWGMT90018_60510)	6119051..6119254	-	204	WP_236978928.1	hypothetical protein	-
L6444_RS28975 (IWGMT90018_60520)	6119230..6119637	-	408	WP_236978930.1	helix-turn-helix domain-containing protein	-
L6444_RS28980 (IWGMT90018_60530)	6119705..6120865	+	1161	WP_236978932.1	LLM class flavin-dependent oxidoreductase	-
L6444_RS28985 (IWGMT90018_60540)	6120887..6121654	-	768	WP_236978934.1	hypothetical protein	-
L6444_RS28990	6122304..6122615	-	312	Protein_5777	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-64)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14417.58 Da
• Isoelectric Point: 6.4792

>T40025 WP_236978924.1 NZ_AP025179:6117283-6117687 [Mycobacterium kiyosense]
VIYLDTSALTKLLIAEAETAALRAWLNAQHERGEHAATSTLGRIELMRAVYRYAATEYVGRARYLLDGLDMVTLSAPVLT
AAETIGPPTLRSLDAIHLAAAAQLKQELTAFVTYDRRLLAGCHDVGIPAISPGS

Nucleotide

• Length: 405 bp

>T40025 NZ_AP025179:6117283-6117687 [Mycobacterium kiyosense]
GTGATCTACCTGGACACCTCGGCCCTGACGAAACTGCTCATCGCCGAGGCTGAGACGGCTGCACTGCGAGCGTGGCTCAA
CGCCCAGCACGAGCGGGGCGAGCACGCAGCGACCAGCACGCTGGGCCGCATCGAGTTGATGCGAGCCGTTTACAGGTACG
CAGCGACTGAGTACGTCGGGCGGGCCCGCTATCTGCTGGACGGCCTTGACATGGTCACGCTCAGCGCACCTGTGTTGACC
GCAGCGGAGACCATCGGTCCGCCTACGCTGCGTTCGCTCGACGCGATCCACCTGGCGGCTGCTGCGCAACTCAAGCAGGA
GCTGACGGCTTTCGTCACTTACGACCGGCGTTTGTTGGCGGGCTGCCACGACGTCGGGATTCCGGCGATATCGCCCGGCT
CATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10102.44 Da
• Isoelectric Point: 5.7202

>AT40025 WP_236978923.1 NZ_AP025179:6117008-6117286 [Mycobacterium kiyosense]
MEVIGVRELRQHASRYLARVEAGEELGVANHGRLVARLVPVAQATRSRTALIDAGLLLPARSPENLYDPAGTSPPSEKRT
LSDVLEEMRDEQ

Nucleotide

• Length: 279 bp

>AT40025 NZ_AP025179:6117008-6117286 [Mycobacterium kiyosense]
ATGGAGGTCATCGGGGTCCGTGAACTGCGACAACATGCATCGCGGTACCTCGCGCGGGTCGAGGCCGGCGAGGAGCTGGG
CGTTGCCAATCACGGGCGCCTGGTTGCCCGCCTCGTACCGGTTGCGCAAGCGACGCGGTCCCGCACCGCCCTGATAGATG
CGGGACTGCTACTCCCGGCCCGCAGCCCGGAAAATCTGTACGACCCCGCCGGCACATCGCCGCCGAGCGAAAAGCGCACA
CTGTCGGACGTTCTCGAAGAAATGCGCGACGAGCAGTGA