Detailed information of TA system TA040022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3447817..3448430
• Replicon: chromosome
• Accession: NZ_AP025179
• Organism: Mycobacterium kiyosense strain IWGMT90018-18076

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L6444_RS16165
• Protein ID: WP_236975464.1
• Coordinates: 3447817..3448221 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L6444_RS16170
• Protein ID: WP_236975466.1
• Coordinates: 3448218..3448430 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6444_RS16150 (IWGMT90018_33820)	3442956..3445256	+	2301	WP_236975451.1	malto-oligosyltrehalose synthase	-
L6444_RS16155 (IWGMT90018_33830)	3445249..3446973	+	1725	WP_236975460.1	malto-oligosyltrehalose trehalohydrolase	-
L6444_RS16160 (IWGMT90018_33840)	3447081..3447668	-	588	WP_236975462.1	hypothetical protein	-
L6444_RS16165 (IWGMT90018_33850)	3447817..3448221	-	405	WP_236975464.1	PIN domain nuclease	Toxin
L6444_RS16170 (IWGMT90018_33860)	3448218..3448430	-	213	WP_236975466.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L6444_RS16175 (IWGMT90018_33870)	3448460..3449209	-	750	WP_236975468.1	peroxide stress protein YaaA	-
L6444_RS16180 (IWGMT90018_33880)	3449286..3449561	+	276	WP_236975470.1	DUF4190 domain-containing protein	-
L6444_RS16185 (IWGMT90018_33890)	3449563..3450888	-	1326	WP_236975478.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
L6444_RS16190 (IWGMT90018_33900)	3450929..3451564	+	636	WP_236975480.1	pyridoxamine 5'-phosphate oxidase family protein	-
L6444_RS16195 (IWGMT90018_33910)	3451581..3452811	-	1231	Protein_3229	threonine ammonia-lyase	-
L6444_RS16200 (IWGMT90018_33920)	3452711..3452926	+	216	WP_236984641.1	hypothetical protein	-
L6444_RS16205 (IWGMT90018_33930)	3452901..3453161	-	261	WP_236975483.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14586.65 Da
• Isoelectric Point: 6.0774

>T40022 WP_236975464.1 NZ_AP025179:c3448221-3447817 [Mycobacterium kiyosense]
VILIDTSAWVEYFRASGSAAAHEVRRLISDHADQLAMCEPVAMEILCGATDDNAQAKLERLVNGLPSLPVDNAIDFRAAA
EVYRSARRVGATIRSITDCLIAAVAIRHGATVMHRDADFDVIARITRLDAQSLR

Nucleotide

• Length: 405 bp

>T40022 NZ_AP025179:c3448221-3447817 [Mycobacterium kiyosense]
GTGATCCTCATCGACACCTCCGCGTGGGTCGAGTACTTTCGGGCCAGCGGATCAGCCGCTGCTCACGAGGTGCGCCGGCT
GATCTCTGACCACGCCGACCAGCTCGCGATGTGCGAGCCGGTGGCTATGGAAATCCTTTGCGGCGCAACCGATGACAACG
CACAGGCAAAACTCGAGCGGTTGGTCAACGGCCTACCCTCACTGCCCGTCGACAACGCCATCGATTTCCGAGCCGCGGCG
GAGGTCTATCGCTCCGCCCGGCGAGTCGGCGCAACGATTCGCAGCATCACCGACTGCCTGATCGCCGCGGTGGCTATCCG
CCATGGCGCTACGGTAATGCACCGCGACGCGGACTTCGACGTCATCGCACGCATCACCCGCCTCGACGCGCAATCGCTGC
GCTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8081.14 Da
• Isoelectric Point: 4.4746

>AT40022 WP_236975466.1 NZ_AP025179:c3448430-3448218 [Mycobacterium kiyosense]
MSRTNIDIDDELAAEVMRRFGLRTKRAAVDLALRRLVGSPLNREFLLELEGVGWEGDLDDLRDDRPDQLR

Nucleotide

• Length: 213 bp

>AT40022 NZ_AP025179:c3448430-3448218 [Mycobacterium kiyosense]
ATGTCGCGCACCAATATCGACATCGACGACGAACTCGCCGCCGAGGTGATGCGTCGGTTCGGCCTGCGCACCAAGAGGGC
CGCCGTCGATCTCGCGCTGCGTCGACTGGTGGGTTCGCCGTTGAATCGCGAGTTCCTGCTCGAGCTCGAAGGTGTCGGCT
GGGAAGGCGACCTCGACGATCTCCGCGACGATCGGCCAGACCAGCTCCGGTGA