Detailed information of TA system TA040011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2127642..2128156
• Replicon: chromosome
• Accession: NZ_AP025177
• Organism: Staphylococcus aureus strain TPS6281

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: CC22TP_RS10525
• Protein ID: WP_000581792.1
• Coordinates: 2127642..2127851 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: CC22TP_RS10530
• Protein ID: WP_000138928.1
• Coordinates: 2127863..2128156 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CC22TP_RS10505 (2123150)	2123150..2124508	-	1359	WP_229346816.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
CC22TP_RS10510 (2124523)	2124523..2125593	-	1071	WP_000159632.1	D-alanine--D-alanine ligase	-
CC22TP_RS10515 (2125911)	2125911..2127113	+	1203	WP_001109944.1	FtsW/RodA/SpoVE family cell cycle protein	-
CC22TP_RS10520 (2127377)	2127377..2127514	-	138	WP_000828354.1	Lmo0850 family protein	-
CC22TP_RS10525 (2127642)	2127642..2127851	-	210	WP_000581792.1	heavy metal-associated domain-containing protein	Toxin
CC22TP_RS10530 (2127863)	2127863..2128156	-	294	WP_000138928.1	copper-sensing transcriptional repressor CsoR	Antitoxin
CC22TP_RS10535 (2128322)	2128322..2129806	+	1485	WP_000571551.1	cardiolipin synthase	-
CC22TP_RS10540 (2129834)	2129834..2130481	+	648	WP_001187617.1	HD domain-containing protein	-
CC22TP_RS10545 (2130959)	2130959..2131831	-	873	WP_000725802.1	membrane protein insertase YidC	-
CC22TP_RS10550 (2131917)	2131917..2132558	-	642	WP_000483153.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T40011 WP_000581792.1 NZ_AP025177:c2127851-2127642 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T40011 NZ_AP025177:c2127851-2127642 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11184.82 Da
• Isoelectric Point: 6.2243

>AT40011 WP_000138928.1 NZ_AP025177:c2128156-2127863 [Staphylococcus aureus]
MTEQDIAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT40011 NZ_AP025177:c2128156-2127863 [Staphylococcus aureus]
ATGACTGAACAAGATATTGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1