Detailed information of TA system TA039995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2170986..2171500
• Replicon: chromosome
• Accession: NZ_AP025176
• Organism: Staphylococcus aureus strain TPS5614

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: LPC25_RS10790
• Protein ID: WP_000581792.1
• Coordinates: 2170986..2171195 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: LPC25_RS10795
• Protein ID: WP_000138928.1
• Coordinates: 2171207..2171500 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPC25_RS10770 (2166494)	2166494..2167852	-	1359	WP_000611469.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
LPC25_RS10775 (2167867)	2167867..2168937	-	1071	WP_000159632.1	D-alanine--D-alanine ligase	-
LPC25_RS10780 (2169255)	2169255..2170457	+	1203	WP_001109944.1	FtsW/RodA/SpoVE family cell cycle protein	-
LPC25_RS10785 (2170721)	2170721..2170858	-	138	WP_000828354.1	Lmo0850 family protein	-
LPC25_RS10790 (2170986)	2170986..2171195	-	210	WP_000581792.1	heavy metal-associated domain-containing protein	Toxin
LPC25_RS10795 (2171207)	2171207..2171500	-	294	WP_000138928.1	copper-sensing transcriptional repressor CsoR	Antitoxin
LPC25_RS10800 (2171666)	2171666..2173150	+	1485	WP_000571551.1	cardiolipin synthase	-
LPC25_RS10805 (2173178)	2173178..2173825	+	648	WP_001187617.1	HD domain-containing protein	-
LPC25_RS10810 (2174303)	2174303..2175175	-	873	WP_000725802.1	membrane protein insertase YidC	-
LPC25_RS10815 (2175261)	2175261..2175902	-	642	WP_000483153.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T39995 WP_000581792.1 NZ_AP025176:c2171195-2170986 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T39995 NZ_AP025176:c2171195-2170986 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11184.82 Da
• Isoelectric Point: 6.2243

>AT39995 WP_000138928.1 NZ_AP025176:c2171500-2171207 [Staphylococcus aureus]
MTEQDIAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT39995 NZ_AP025176:c2171500-2171207 [Staphylococcus aureus]
ATGACTGAACAAGATATTGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1