Detailed information of TA system TA039986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 816801..817330
• Replicon: chromosome
• Accession: NZ_AP025176
• Organism: Staphylococcus aureus strain TPS5614

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: K7ZRX6
• Locus tag: LPC25_RS03815
• Protein ID: WP_001103939.1
• Coordinates: 817013..817330 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IY59
• Locus tag: LPC25_RS03810
• Protein ID: WP_001058494.1
• Coordinates: 816801..817010 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPC25_RS03775 (813131)	813131..813595	+	465	WP_001085185.1	SsrA-binding protein SmpB	-
LPC25_RS03785 (814158)	814158..815264	-	1107	WP_000149511.1	tyrosine-type recombinase/integrase	-
LPC25_RS03790 (815254)	815254..816057	-	804	WP_000358990.1	helix-turn-helix transcriptional regulator	-
LPC25_RS03795 (816193)	816193..816396	+	204	WP_001045296.1	transcriptional regulator	-
LPC25_RS03800 (816432)	816432..816650	+	219	WP_000163544.1	helix-turn-helix transcriptional regulator	-
LPC25_RS03805 (816662)	816662..816808	+	147	WP_000784885.1	hypothetical protein	-
LPC25_RS03810 (816801)	816801..817010	+	210	WP_001058494.1	hypothetical protein	Antitoxin
LPC25_RS03815 (817013)	817013..817330	+	318	WP_001103939.1	DUF1474 family protein	Toxin
LPC25_RS03820 (817394)	817394..818263	+	870	WP_001002717.1	primase alpha helix C-terminal domain-containing protein	-
LPC25_RS03825 (818280)	818280..819749	+	1470	WP_069994633.1	virulence-associated E family protein	-
LPC25_RS03830 (820035)	820035..820397	+	363	WP_049317376.1	hypothetical protein	-
LPC25_RS03835 (820399)	820399..820683	+	285	WP_000998179.1	hypothetical protein	-
LPC25_RS03840 (820680)	820680..821321	+	642	WP_031765326.1	pathogenicity island protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	tsst-1 / sec / sell / vWbp	810737..849937	39200

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12547.17 Da
• Isoelectric Point: 5.0161

>T39986 WP_001103939.1 NZ_AP025176:817013-817330 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKQKINDVATKHAWFVEDRFVKNELETKREHINFSASYLEHRIQNEHTVELLHVYLKEFGELIQKF
HEIEKASSENFGEVSDDAQKLKITE

Nucleotide

• Length: 318 bp

>T39986 NZ_AP025176:817013-817330 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGTATCAGATGACGCACAAAAATTAAAAATCACAGAGTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8268.31 Da
• Isoelectric Point: 4.3718

>AT39986 WP_001058494.1 NZ_AP025176:816801-817010 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNSIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT39986 NZ_AP025176:816801-817010 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATAGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: K7ZRX6

Antitoxin

• AlphaFold DB: X5IY59