Detailed information of TA system TA039985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 877247..878283
• Replicon: plasmid pMTS1
• Accession: NZ_AP025172
• Organism: Cupriavidus sp. P-10

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CTP10_RS34020
• Protein ID: WP_116323977.1
• Coordinates: 877247..877819 (-)
• Length: 191 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A388S344
• Locus tag: CTP10_RS34025
• Protein ID: WP_039014334.1
• Coordinates: 877822..878283 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTP10_RS34010 (CTP10_R67940)	875492..876505	-	1014	WP_116324091.1	IS110 family transposase	-
CTP10_RS34015 (CTP10_R67950)	876529..877032	+	504	WP_116323978.1	nuclear transport factor 2 family protein	-
CTP10_RS34020 (CTP10_R67960)	877247..877819	-	573	WP_116323977.1	PIN domain-containing protein	Toxin
CTP10_RS34025 (CTP10_R67970)	877822..878283	-	462	WP_039014334.1	excisionase family DNA-binding protein	Antitoxin
CTP10_RS34030 (CTP10_R67980)	878757..879350	-	594	WP_116323976.1	hypothetical protein	-
CTP10_RS34035	879347..881281	-	1935	Protein_789	alkyl sulfatase dimerization domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG / htpB	1..1635938	1635938
	-	flank	IS/Tn	-	-	875492..876505	1013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-114)

Antitoxin

• Helix-turn-helix domain, group 17 (83-130)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 21152.22 Da
• Isoelectric Point: 5.5772

>T39985 WP_116323977.1 NZ_AP025172:c877819-877247 [Cupriavidus sp. P-10]
MASNFAVVYDACVLYPAPLRDLLMRLALTDLYRAKWTNEIHDEWIRNLLARRPELSPDRLAGTRELMNRSVRDSLISGYE
HLIGSIKLPDPDDRHVVAAAIHSGSEAIITFNLKDFPEAALKEFNVEAIHPDDFVVDLFDLNAGKVLAAVAEHRASLKNP
PKSASEYLTTLSAQGLTQTVLILTQYSLAI

Nucleotide

• Length: 573 bp

>T39985 NZ_AP025172:c877819-877247 [Cupriavidus sp. P-10]
ATGGCTTCTAACTTCGCTGTAGTCTACGATGCCTGCGTTCTCTATCCTGCGCCGCTGCGGGATCTGCTGATGCGTCTCGC
TCTCACCGACCTGTACCGTGCAAAGTGGACAAATGAAATTCATGACGAGTGGATTCGCAACCTCCTCGCGCGGCGGCCGG
AGCTCTCCCCGGATCGCCTTGCCGGCACGCGCGAACTGATGAACCGAAGTGTTCGCGATTCGCTGATTAGCGGGTATGAG
CACCTTATTGGGTCCATTAAGCTGCCTGACCCTGACGATCGACATGTGGTTGCGGCCGCAATTCATAGCGGCTCTGAGGC
AATCATCACGTTTAATTTGAAGGACTTCCCCGAAGCCGCACTCAAGGAGTTCAACGTCGAGGCGATCCACCCGGATGATT
TCGTCGTGGACCTCTTTGATCTGAATGCAGGGAAGGTCCTAGCGGCAGTGGCGGAACACCGTGCGTCTCTGAAAAATCCG
CCAAAGTCTGCTTCGGAGTATTTGACTACCCTTTCGGCACAAGGCCTGACGCAAACGGTGTTGATCCTCACGCAGTACAG
TCTGGCAATTTGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17059.26 Da
• Isoelectric Point: 5.2648

>AT39985 WP_039014334.1 NZ_AP025172:c878283-877822 [Cupriavidus sp. P-10]
MHATITNTTLPSANESAIARESSRSLAVALDSRSETQQFDFKTDKGELHSVTLPTSALRLLVEVLAEIGQGNAVSIIPIH
AELTTQEAADLLNVSRPYLVQLLEKGEIPFRKVNTHRRVLYQDVVSYKQRIDDERRKALDELAAQAQELDMGY

Nucleotide

• Length: 462 bp

>AT39985 NZ_AP025172:c878283-877822 [Cupriavidus sp. P-10]
ATGCACGCAACCATCACCAACACGACGCTGCCTTCGGCAAACGAAAGTGCTATCGCCCGTGAATCGAGCCGCAGCCTGGC
GGTAGCCTTGGACAGCCGCTCCGAGACGCAGCAATTCGACTTCAAGACGGACAAGGGCGAGTTGCATAGTGTCACATTGC
CCACCTCGGCGTTGAGGCTTCTGGTCGAGGTTCTCGCTGAAATCGGCCAAGGGAACGCCGTCTCTATCATCCCCATTCAC
GCCGAACTGACGACGCAAGAGGCGGCGGACCTGCTTAACGTCTCTCGGCCGTACTTGGTTCAGCTTCTAGAGAAGGGGGA
GATTCCGTTCCGCAAAGTCAACACGCATCGGCGCGTCCTCTACCAAGACGTTGTTAGCTACAAGCAGCGCATTGATGACG
AGCGACGTAAGGCACTAGACGAACTCGCGGCGCAGGCCCAAGAGCTGGACATGGGCTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A388S344