Detailed information of TA system TA039977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2528497..2529135
• Replicon: chromosome
• Accession: NZ_AP025169
• Organism: Pseudomonas sp. CYM-20-01

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: M2497_RS11555
• Protein ID: WP_081049342.1
• Coordinates: 2528497..2528682 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0W0H9Q9
• Locus tag: M2497_RS11560
• Protein ID: WP_016977415.1
• Coordinates: 2528734..2529135 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2497_RS11530 (cym2001_22620)	2523972..2524244	-	273	WP_071495081.1	DUF1652 domain-containing protein	-
M2497_RS11535 (cym2001_22630)	2524347..2525843	-	1497	WP_248487570.1	helix-turn-helix transcriptional regulator	-
M2497_RS11540 (cym2001_22640)	2526015..2526635	+	621	WP_033902081.1	lipoprotein	-
M2497_RS11545 (cym2001_22650)	2526672..2527559	-	888	WP_248487572.1	nitrilase family protein	-
M2497_RS11550 (cym2001_22660)	2527711..2527956	-	246	WP_016976709.1	DUF3077 domain-containing protein	-
M2497_RS11555	2528497..2528682	+	186	WP_081049342.1	type II toxin-antitoxin system HicA family toxin	Toxin
M2497_RS11560 (cym2001_22670)	2528734..2529135	+	402	WP_016977415.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
M2497_RS11565 (cym2001_22680)	2529175..2529519	+	345	WP_248487574.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
M2497_RS11570 (cym2001_22690)	2529966..2532671	+	2706	WP_248487576.1	magnesium-translocating P-type ATPase	-
M2497_RS11575 (cym2001_22700)	2532688..2533404	+	717	WP_058427147.1	MgtC/SapB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6778.94 Da
• Isoelectric Point: 11.1475

>T39977 WP_081049342.1 NZ_AP025169:2528497-2528682 [Pseudomonas sp. CYM-20-01]
MRSREVIDLLVADGWVEVAVKGSHHQFKHPVKRGRVTVPHPKAEIAKGTLHSIFKSAGLKQ

Nucleotide

• Length: 186 bp

>T39977 NZ_AP025169:2528497-2528682 [Pseudomonas sp. CYM-20-01]
ATGAGAAGTCGAGAGGTGATTGACCTGTTGGTTGCAGATGGTTGGGTTGAGGTGGCGGTGAAGGGGAGTCATCACCAGTT
CAAACACCCTGTGAAGCGGGGGAGAGTAACCGTCCCTCATCCCAAGGCGGAGATTGCCAAGGGCACTCTACACAGTATTT
TCAAGTCAGCAGGGCTCAAGCAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14506.22 Da
• Isoelectric Point: 4.5928

>AT39977 WP_016977415.1 NZ_AP025169:2528734-2529135 [Pseudomonas sp. CYM-20-01]
MKFPVVVHKDADSDYSVTVPDVPGCFSAGGSFSEALDNVREALALHLEGLVADQEQLPQAQEVDAHIGNPDFAGGVWAII
DFDLTPYLGKSVRFNASLPEHLLQRIDERVQKDHRYASRSGFLATAALRELSA

Nucleotide

• Length: 402 bp

>AT39977 NZ_AP025169:2528734-2529135 [Pseudomonas sp. CYM-20-01]
ATGAAATTCCCAGTGGTAGTGCATAAAGACGCTGACTCGGATTACAGCGTGACCGTTCCTGATGTGCCGGGGTGTTTCTC
GGCGGGTGGATCTTTCTCCGAAGCATTGGATAATGTTCGTGAAGCATTGGCTTTGCACCTTGAAGGCCTGGTTGCCGATC
AAGAACAGCTTCCTCAGGCGCAGGAGGTTGATGCTCATATAGGTAATCCGGATTTCGCAGGTGGTGTCTGGGCGATCATT
GACTTTGATCTAACGCCCTATCTGGGCAAGTCAGTGCGGTTTAATGCCTCTTTGCCGGAACATCTGTTGCAACGCATTGA
TGAGCGTGTGCAGAAGGACCATCGCTATGCATCGCGGTCAGGGTTCCTGGCGACTGCTGCATTGCGCGAGCTTTCAGCGT
AA

Structures

Antitoxin

• AlphaFold DB: A0A0W0H9Q9