Detailed information of TA system TA039976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2240764..2241368
• Replicon: chromosome
• Accession: NZ_AP025169
• Organism: Pseudomonas sp. CYM-20-01

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1V2J6Y1
• Locus tag: M2497_RS10175
• Protein ID: WP_071496250.1
• Coordinates: 2240764..2240952 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: M2497_RS10180
• Protein ID: WP_248487226.1
• Coordinates: 2240964..2241368 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2497_RS10150 (cym2001_20020)	2236500..2237222	+	723	WP_248487222.1	phosphonate C-P lyase system protein PhnL	-
M2497_RS10155 (cym2001_20030)	2237212..2238357	+	1146	WP_248487223.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
M2497_RS10160 (cym2001_20040)	2238357..2238929	+	573	WP_248487224.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
M2497_RS10165 (cym2001_20050)	2238920..2239669	+	750	WP_248487225.1	phosphonate metabolism protein PhnP	-
M2497_RS10170 (cym2001_20060)	2239692..2240573	-	882	WP_071496251.1	LysR family transcriptional regulator	-
M2497_RS10175 (cym2001_20070)	2240764..2240952	+	189	WP_071496250.1	type II toxin-antitoxin system HicA family toxin	Toxin
M2497_RS10180 (cym2001_20080)	2240964..2241368	+	405	WP_248487226.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
M2497_RS10185 (cym2001_20090)	2241751..2243274	+	1524	WP_248487227.1	M10 family metallopeptidase C-terminal domain-containing protein	-
M2497_RS10190 (cym2001_20100)	2243277..2244482	-	1206	WP_248487228.1	MFS transporter	-
M2497_RS10195 (cym2001_20110)	2244658..2245272	+	615	WP_093123204.1	DUF5666 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7179.35 Da
• Isoelectric Point: 10.8676

>T39976 WP_071496250.1 NZ_AP025169:2240764-2240952 [Pseudomonas sp. CYM-20-01]
VQSRLLIKELQEAGWVLDRVTGSHHIFTHRYSPYTIPVPHPKKDLPLGTVRSIRKRAGLFDF

Nucleotide

• Length: 189 bp

>T39976 NZ_AP025169:2240764-2240952 [Pseudomonas sp. CYM-20-01]
GTGCAAAGCAGGCTATTGATCAAGGAACTGCAAGAGGCGGGTTGGGTATTGGATCGGGTCACGGGCAGCCATCACATTTT
TACCCATCGTTACAGCCCGTACACGATTCCCGTGCCTCATCCTAAAAAAGACTTGCCGTTGGGCACTGTCAGAAGCATCA
GGAAACGCGCCGGGCTGTTTGATTTTTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14724.71 Da
• Isoelectric Point: 4.6000

>AT39976 WP_248487226.1 NZ_AP025169:2240964-2241368 [Pseudomonas sp. CYM-20-01]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGAIPMPTSVAHYHAHEDYAGMGWGM
LELDVTPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT39976 NZ_AP025169:2240964-2241368 [Pseudomonas sp. CYM-20-01]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATCCAGATCCCCGATATTCCAGGCGCAGT
CACCGCCGGGGACAGCTTTGAAGAGGCCTACAACGCCGCCGTGGAAGTCGCGCACATCATGCTGCAAGAGATCGCCGCAG
AGGGCGGGGCCATTCCGATGCCAACGTCGGTCGCCCATTATCATGCCCATGAGGACTACGCCGGGATGGGCTGGGGCATG
TTGGAGTTGGACGTCACGCCCTACCTGGGTAAAACCGAGAAGGTCAATGTGACCTTGCCCGGTTATGTCATCCAGCGCAT
CGACCGGTATGTACGGGAGCACAAGGTCAAAAGCCGCTCGTCATTCCTCGCGGACGCGGCGTTGGAAAAATTGGTGCGGT
CCTAG

Structures

Toxin

• AlphaFold DB: A0A1V2J6Y1