Detailed information of TA system TA039975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TacT2-ataR/DUF1778(antitoxin)
• Location: 1896441..1897216
• Replicon: chromosome
• Accession: NZ_AP025169
• Organism: Pseudomonas sp. CYM-20-01

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: M2497_RS08470
• Protein ID: WP_248486836.1
• Coordinates: 1896441..1896938 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: M2497_RS08475
• Protein ID: WP_248486839.1
• Coordinates: 1896938..1897216 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2497_RS08445 (cym2001_16610)	1892488..1892829	-	342	WP_248486830.1	hypothetical protein	-
M2497_RS08450 (cym2001_16620)	1892831..1894147	-	1317	WP_248488182.1	integrating conjugative element protein	-
M2497_RS08455 (cym2001_16630)	1894190..1895122	-	933	WP_248486831.1	TIGR03756 family integrating conjugative element protein	-
M2497_RS08460 (cym2001_16640)	1895329..1895823	+	495	WP_248486832.1	DUF2165 domain-containing protein	-
M2497_RS08465 (cym2001_16650)	1895888..1896187	-	300	WP_248486834.1	hypothetical protein	-
M2497_RS08470 (cym2001_16660)	1896441..1896938	-	498	WP_248486836.1	GNAT family N-acetyltransferase	Toxin
M2497_RS08475 (cym2001_16670)	1896938..1897216	-	279	WP_248486839.1	DUF1778 domain-containing protein	Antitoxin
M2497_RS08480	1897280..1897783	-	504	WP_248486840.1	DNA repair protein RadC	-
M2497_RS08485 (cym2001_16690)	1898324..1899352	-	1029	WP_248486841.1	restriction endonuclease	-
M2497_RS08490	1899457..1900605	-	1149	WP_248486842.1	hypothetical protein	-
M2497_RS08495 (cym2001_16710)	1900773..1901426	-	654	WP_248486843.1	DUF2971 domain-containing protein	-
M2497_RS08500 (cym2001_16720)	1901527..1901838	-	312	WP_169892602.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1888455..1950657	62202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-84)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18174.95 Da
• Isoelectric Point: 9.6826

>T39975 WP_248486836.1 NZ_AP025169:c1896938-1896441 [Pseudomonas sp. CYM-20-01]
MLRAPQPLDASHVLEPFDCGKPTLNEWLIRHARQAQSSGSARTFVVLDDERLVGYFSLTVGQVDVLTAPERISRGMGRYP
IPVAILARLAVSQTDQGRGIGVGLLRDAIVRTLTVADQVGVRALLTHPLDDEAARFYRRFGFESSPASESQLLLLLKDAR
KLLAR

Nucleotide

• Length: 498 bp

>T39975 NZ_AP025169:c1896938-1896441 [Pseudomonas sp. CYM-20-01]
ATGCTTCGAGCACCGCAGCCGCTGGACGCCAGCCATGTGCTGGAACCCTTCGACTGCGGCAAACCCACGCTCAACGAGTG
GCTTATCCGCCACGCCCGGCAAGCACAGTCCAGTGGTTCAGCCAGGACCTTCGTGGTACTGGACGACGAGCGACTGGTCG
GGTACTTCAGCCTGACCGTCGGCCAGGTTGATGTCCTCACCGCCCCGGAGCGGATCAGCCGCGGCATGGGCCGCTATCCC
ATCCCCGTGGCTATCCTTGCCCGTCTGGCGGTATCGCAGACCGATCAGGGGCGCGGTATCGGCGTTGGTCTGCTGCGCGA
TGCCATCGTTCGCACATTGACGGTGGCCGACCAGGTCGGTGTGCGAGCCTTGCTGACTCATCCGCTGGATGACGAGGCGG
CACGCTTCTACCGGCGGTTCGGCTTCGAATCCTCGCCGGCCAGCGAATCGCAACTGCTGTTGCTGCTCAAAGATGCGCGC
AAGCTGCTCGCACGTTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10232.65 Da
• Isoelectric Point: 4.9611

>AT39975 WP_248486839.1 NZ_AP025169:c1897216-1896938 [Pseudomonas sp. CYM-20-01]
MANPSSRSARLGLRTTLEQDIVLRRAAKLANKSLTDFILDSAYQVAEQTLLDQRLFLLDGVQAQAFVDLLDRPAQDNPGL
QDLLGRPIPWSN

Nucleotide

• Length: 279 bp

>AT39975 NZ_AP025169:c1897216-1896938 [Pseudomonas sp. CYM-20-01]
ATGGCTAATCCCTCCTCCCGCAGTGCCCGGCTTGGCCTGCGCACCACCCTGGAGCAGGACATCGTTCTGCGCCGGGCTGC
CAAGCTGGCCAACAAATCGTTGACCGACTTCATCCTCGACAGCGCCTATCAGGTCGCCGAGCAGACTCTGCTCGACCAAC
GCCTGTTCTTGCTAGATGGGGTACAGGCCCAGGCCTTCGTCGACCTGCTCGACCGGCCCGCTCAGGACAATCCCGGCTTA
CAAGACCTGCTGGGCCGTCCTATTCCCTGGAGCAACTGA