Detailed information of TA system TA039973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1839626..1840241
• Replicon: chromosome
• Accession: NZ_AP025169
• Organism: Pseudomonas sp. CYM-20-01

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: M2497_RS08190
• Protein ID: WP_248486783.1
• Coordinates: 1840059..1840241 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1V2JLU3
• Locus tag: M2497_RS08185
• Protein ID: WP_071496822.1
• Coordinates: 1839626..1840036 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2497_RS08160 (cym2001_16080)	1834779..1835972	+	1194	WP_248486776.1	CaiB/BaiF CoA-transferase family protein	-
M2497_RS08165 (cym2001_16090)	1835969..1836910	+	942	WP_248486777.1	hydroxymethylglutaryl-CoA lyase	-
M2497_RS08170 (cym2001_16100)	1836970..1838301	+	1332	WP_248486779.1	MFS transporter	-
M2497_RS08175 (cym2001_16110)	1838442..1838969	+	528	WP_016975135.1	DUF4142 domain-containing protein	-
M2497_RS08180 (cym2001_16120)	1839035..1839535	-	501	WP_248486781.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
M2497_RS08185 (cym2001_16130)	1839626..1840036	-	411	WP_071496822.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
M2497_RS08190 (cym2001_16140)	1840059..1840241	-	183	WP_248486783.1	type II toxin-antitoxin system HicA family toxin	Toxin
M2497_RS08195 (cym2001_16150)	1840410..1841303	-	894	WP_248486785.1	LysR family transcriptional regulator	-
M2497_RS08200 (cym2001_16160)	1841399..1842562	+	1164	WP_248486788.1	MFS transporter	-
M2497_RS08205 (cym2001_16170)	1842559..1843728	+	1170	WP_248486790.1	MFS transporter	-
M2497_RS08210	1843916..1844230	+	315	WP_248488179.1	hypothetical protein	-
M2497_RS08215 (cym2001_16180)	1844230..1844523	+	294	WP_248488180.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6763.99 Da
• Isoelectric Point: 10.7532

>T39973 WP_248486783.1 NZ_AP025169:c1840241-1840059 [Pseudomonas sp. CYM-20-01]
VDSRYLIGHIVADGWYLVRIRGSHHPFKHPTKPGLVTIPHPKKDLLDKTAKSILKQALLS

Nucleotide

• Length: 183 bp

>T39973 NZ_AP025169:c1840241-1840059 [Pseudomonas sp. CYM-20-01]
GTGGATAGCCGATATTTGATAGGTCACATCGTTGCGGACGGTTGGTATCTGGTACGTATCAGAGGCAGCCACCACCCCTT
CAAGCACCCGACAAAACCGGGGCTGGTGACGATTCCCCATCCAAAGAAGGACCTGTTGGACAAGACCGCCAAGAGCATTT
TGAAACAGGCTCTGCTGAGTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14782.64 Da
• Isoelectric Point: 4.4921

>AT39973 WP_071496822.1 NZ_AP025169:c1840036-1839626 [Pseudomonas sp. CYM-20-01]
MLYPIAISTGDDDHAWGVEVPDIPGCYSAGDDLDDAMAMAREAIEGHFEILAEDGAPIPSAQKVTLHAANPHYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQDH

Nucleotide

• Length: 411 bp

>AT39973 NZ_AP025169:c1840036-1839626 [Pseudomonas sp. CYM-20-01]
ATGCTTTACCCGATTGCAATTTCAACCGGCGACGACGACCACGCCTGGGGCGTAGAAGTCCCGGATATTCCAGGCTGCTA
CTCCGCCGGCGACGACTTGGACGACGCCATGGCCATGGCTCGCGAAGCCATTGAAGGACATTTCGAAATCCTGGCGGAAG
ACGGTGCACCGATTCCCAGTGCGCAAAAGGTCACCTTGCACGCCGCCAACCCTCACTATGCAGGTTGCACCTGGGCGGTG
GTGGACATTGATGTCACCAAGTACCTGGGCAAGGCGCAGAAGCTCAACATCACGCTGCCCGGTTATCTGTTGAACCGCAT
CGATGAATATGTGCTGCACCACCCGGAAGAAAAAAGCCGTTCCGGCTTTCTGGCCTCAGCGGCGCTTAAAGTGTTGCAGC
AAGACCACTGA

Structures

Antitoxin

• AlphaFold DB: A0A1V2JLU3