Detailed information of TA system TA039971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 657..1858
• Replicon: plasmid KP-1
• Accession: NZ_AP025163
• Organism: Lactobacillus crispatus strain KT-11

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: LPC26_RS11715
• Protein ID: WP_048344620.1
• Coordinates: 657..1583 (-)
• Length: 309 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: -
• Locus tag: LPC26_RS11720
• Protein ID: WP_024305851.1
• Coordinates: 1586..1858 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPC26_RS11705	136..363	+	228	WP_048344618.1	hypothetical protein	-
LPC26_RS11710	389..664	+	276	WP_048344619.1	hypothetical protein	-
LPC26_RS11715	657..1583	-	927	WP_048344620.1	zeta toxin family protein	Toxin
LPC26_RS11720	1586..1858	-	273	WP_024305851.1	epsilon-antitoxin	Antitoxin
LPC26_RS11725	2163..3272	-	1110	WP_229265848.1	replication initiator protein A	-
LPC26_RS11730	3819..4094	-	276	WP_229265849.1	dihydrodipicolinate reductase	-
LPC26_RS11735	4097..5146	-	1050	WP_229265850.1	ParM/StbA family protein	-
LPC26_RS11740	5499..6185	-	687	WP_229265851.1	DUF3592 domain-containing protein	-
LPC26_RS11745	6425..6706	-	282	Protein_8	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34332	34332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (24-213)

Antitoxin

• Bacterial epsilon antitoxin (4-90)

Sequences

Toxin

Protein

• Length: 309 a.a.
• Molecular weight: 35593.29 Da
• Isoelectric Point: 6.2635

>T39971 WP_048344620.1 NZ_AP025163:c1583-657 [Lactobacillus crispatus]
MDELADYTDHQFETKLQIEIEQLTKNKQAQASPKAYLLGGQPGAGKSGLHQLIKAQDPNAITIDNDTFKWLHPKYQQLEQ
KYGKDVVKYVTPFSNRMTESLIDYLSDKKFDLIIEGTLRTVEVPMATVTKLQNRGYEASLYVMAVPRIESYLGTLARYED
QFSLSPKTARATTKEAHDLVVRQLPDNLDFLYKQRLFKEIRLYDRKGNKLYSSLENLNESPKQIITEILNRKLDNNTLLN
SIDSVINKMEINHHTTTPQYLDLVEKSTDLKKEISDEVQAQLKEFAEQNPDVKQPENKQEQKDQPRLN

Nucleotide

• Length: 927 bp

>T39971 NZ_AP025163:c1583-657 [Lactobacillus crispatus]
ATGGACGAACTAGCTGACTATACCGATCATCAATTTGAAACGAAATTACAGATTGAAATCGAGCAATTAACCAAAAACAA
GCAAGCCCAGGCTAGTCCCAAAGCTTATTTACTAGGAGGACAACCAGGAGCAGGAAAATCAGGGTTGCACCAACTTATAA
AGGCACAAGATCCTAATGCGATTACGATTGATAACGATACTTTTAAATGGCTACACCCAAAATACCAACAATTAGAGCAA
AAATACGGGAAAGATGTTGTAAAATATGTAACCCCGTTTTCTAACCGAATGACTGAATCTTTAATTGATTATCTGAGTGA
TAAAAAATTTGATTTAATTATCGAAGGGACGTTAAGAACAGTTGAAGTACCAATGGCCACTGTAACCAAATTACAGAATA
GGGGTTACGAAGCGAGTTTATACGTTATGGCAGTTCCGAGAATTGAATCATATCTAGGGACTTTGGCACGCTATGAGGAT
CAATTTAGTTTATCACCTAAAACTGCTAGAGCGACTACCAAAGAAGCTCATGATCTCGTTGTCAGGCAGCTGCCTGATAA
TTTAGATTTTCTGTATAAACAGAGGCTGTTTAAAGAGATCAGACTATATGACCGAAAGGGTAACAAATTATATTCTAGCC
TAGAAAACTTGAACGAAAGCCCCAAACAGATTATTACTGAAATTCTTAACAGAAAGCTAGATAATAATACCCTGCTCAAT
AGCATAGATAGTGTTATTAATAAAATGGAGATTAATCATCATACAACTACGCCGCAGTACCTCGATTTAGTAGAAAAATC
AACAGATTTAAAAAAAGAGATCAGCGATGAGGTACAAGCACAATTGAAAGAATTTGCTGAACAAAATCCAGACGTTAAGC
AACCAGAAAATAAGCAAGAACAAAAAGACCAACCTAGACTTAACTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10329.89 Da
• Isoelectric Point: 5.0356

>AT39971 WP_024305851.1 NZ_AP025163:c1858-1586 [Lactobacillus crispatus]
MKLDYQQTYKKEILTEFASSIYAKVVNLVVDQELDIHDESHFLVKLMHQLGDAKLAIMDAHSLGELETIQAYWQAMNNFI
DSLLTKSKVA

Nucleotide

• Length: 273 bp

>AT39971 NZ_AP025163:c1858-1586 [Lactobacillus crispatus]
ATGAAATTAGACTATCAACAAACTTACAAAAAGGAAATTCTAACCGAATTTGCCAGCAGCATTTATGCTAAAGTAGTCAA
TTTAGTTGTTGACCAAGAATTAGATATTCACGATGAAAGTCATTTTCTAGTTAAACTTATGCATCAGTTAGGAGATGCTA
AGTTAGCCATTATGGACGCGCATTCATTGGGAGAGTTAGAGACAATTCAAGCTTACTGGCAAGCAATGAATAACTTTATT
GATAGCCTGCTAACGAAAAGTAAAGTCGCTTAG