Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/YoeB-RelB
Location 2185881..2186410 Replicon chromosome
Accession NZ_AP025162
Organism Lactobacillus crispatus strain KT-11

Toxin (Protein)


Gene name Txe Uniprot ID A0A854ZB67
Locus tag LPC26_RS10775 Protein ID WP_025006274.1
Coordinates 2185881..2186138 (-) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID V5E284
Locus tag LPC26_RS10780 Protein ID WP_013087009.1
Coordinates 2186135..2186410 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPC26_RS10750 2181271..2182770 - 1500 WP_023487729.1 glycerol kinase GlpK -
LPC26_RS10755 2182895..2183710 + 816 WP_005720042.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
LPC26_RS10760 2183765..2184037 - 273 WP_060461798.1 hypothetical protein -
LPC26_RS10765 2184039..2184581 - 543 WP_060461799.1 hypothetical protein -
LPC26_RS10770 2184945..2185778 - 834 WP_060461801.1 helix-turn-helix transcriptional regulator -
LPC26_RS10775 2185881..2186138 - 258 WP_025006274.1 Txe/YoeB family addiction module toxin Toxin
LPC26_RS10780 2186135..2186410 - 276 WP_013087009.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LPC26_RS10785 2186604..2186735 - 132 WP_041811095.1 hypothetical protein -
LPC26_RS10790 2186847..2187257 + 411 WP_013087010.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
LPC26_RS10795 2187263..2187556 + 294 WP_060461802.1 type II toxin-antitoxin system RelE/ParE family toxin -
LPC26_RS10800 2187626..2188312 + 687 WP_060461803.1 cobalamin biosynthesis protein CobQ -
LPC26_RS10805 2188358..2188843 - 486 WP_005721846.1 C40 family peptidase -
LPC26_RS10810 2189038..2189388 + 351 WP_013087014.1 hypothetical protein -
LPC26_RS10815 2189510..2190385 + 876 WP_060461804.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10219.64 Da        Isoelectric Point: 8.9406

>T39970 WP_025006274.1 NZ_AP025162:c2186138-2185881 [Lactobacillus crispatus]
MIVAWTDDGWNDYVYWYDDGDYKKVSRINDLVKDMKRHPFTGIGKPEPLKRNLSGLWSRRIDSKNRIVYDCHKSMITIYS
CKDHY

Download         Length: 258 bp

>T39970 NZ_AP025162:c2186138-2185881 [Lactobacillus crispatus]
ATGATCGTAGCATGGACGGATGATGGTTGGAACGACTATGTGTATTGGTATGACGATGGTGATTATAAAAAGGTATCAAG
AATAAACGATCTTGTGAAAGATATGAAAAGACATCCATTTACAGGTATTGGTAAGCCTGAGCCCTTAAAGAGAAATCTAT
CAGGGTTATGGTCAAGACGAATCGATTCGAAAAATCGGATCGTCTATGATTGTCATAAATCAATGATAACTATATATTCA
TGTAAGGATCACTATTGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10597.82 Da        Isoelectric Point: 4.5716

>AT39970 WP_013087009.1 NZ_AP025162:c2186410-2186135 [Lactobacillus crispatus]
MDAVNYSNFRKNLKHYFKQVNDDSEPLIVTNKEPEDNVVVLGKEDYDALVETLEIQSNQYLMDKIERGRKQVSEGTIKQH
ELIDPEQVTKS

Download         Length: 276 bp

>AT39970 NZ_AP025162:c2186410-2186135 [Lactobacillus crispatus]
ATGGATGCGGTTAATTACAGTAATTTTCGTAAAAATTTAAAGCATTATTTTAAGCAAGTCAATGATGATAGTGAACCTTT
GATCGTAACTAATAAAGAACCAGAGGATAATGTGGTTGTATTAGGTAAAGAAGATTATGATGCCTTGGTTGAAACATTAG
AAATTCAATCTAATCAATACCTAATGGACAAGATTGAAAGAGGGCGTAAACAAGTTAGTGAAGGCACGATAAAGCAACAT
GAACTAATTGATCCTGAGCAGGTAACAAAATCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A854ZB67

Antitoxin

Source ID Structure
AlphaFold DB V5E284

References