Detailed information of TA system TA039969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 2793002..2793638
• Replicon: chromosome
• Accession: NZ_AP025160
• Organism: Acidithiobacillus ferrooxidans strain NFP31

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B7J980
• Locus tag: ANFP_RS14300
• Protein ID: WP_012537455.1
• Coordinates: 2793002..2793418 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7J981
• Locus tag: ANFP_RS14305
• Protein ID: WP_009560865.1
• Coordinates: 2793405..2793638 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANFP_RS14270 (ANFP_28700)	2788341..2788628	-	288	WP_012537449.1	DDE-type integrase/transposase/recombinase	-
ANFP_RS14275 (ANFP_28710)	2788991..2789917	-	927	WP_012537450.1	uracil-DNA glycosylase	-
ANFP_RS14280 (ANFP_28720)	2790084..2791154	+	1071	WP_012537451.1	linear amide C-N hydrolase	-
ANFP_RS14285 (ANFP_28730)	2791367..2791732	-	366	WP_257245758.1	type II toxin-antitoxin system PemK/MazF family toxin	-
ANFP_RS14290 (ANFP_28740)	2791747..2791998	-	252	WP_012537453.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
ANFP_RS14295	2792318..2792899	+	582	WP_146235847.1	hypothetical protein	-
ANFP_RS14300 (ANFP_28760)	2793002..2793418	-	417	WP_012537455.1	PIN domain-containing protein	Toxin
ANFP_RS14305 (ANFP_28770)	2793405..2793638	-	234	WP_009560865.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ANFP_RS14310 (ANFP_28780)	2793637..2793897	+	261	WP_012607558.1	hypothetical protein	-
ANFP_RS14315 (ANFP_28790)	2793994..2794344	+	351	WP_012537457.1	DUF4870 domain-containing protein	-
ANFP_RS14320 (ANFP_28800)	2794441..2796303	+	1863	WP_012537458.1	ATP-binding cassette domain-containing protein	-
ANFP_RS14325 (ANFP_28810)	2796300..2796623	-	324	WP_012537459.1	hypothetical protein	-
ANFP_RS14330 (ANFP_28820)	2796616..2796927	-	312	WP_009566855.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-119)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15358.75 Da
• Isoelectric Point: 5.1352

>T39969 WP_012537455.1 NZ_AP025160:c2793418-2793002 [Acidithiobacillus ferrooxidans]
MASGKAFFDTNVLLYLLSADTAKADVAEGIMVNGGVISAQVLNEFASVAQRKMGMSFLEIREVLTPIHRICDVAHLTLDT
HIHGLRIAERYKYNVWDAMIVASALLADCAVLYTEDMHHGQILEDRLQLINPFLRADE

Nucleotide

• Length: 417 bp

>T39969 NZ_AP025160:c2793418-2793002 [Acidithiobacillus ferrooxidans]
ATGGCGAGCGGTAAGGCGTTCTTCGACACGAACGTGCTGCTTTATCTGCTTTCGGCGGACACGGCCAAGGCAGATGTTGC
AGAAGGCATCATGGTGAATGGTGGTGTGATCAGTGCCCAGGTGTTGAACGAATTTGCTTCCGTTGCCCAGCGCAAGATGG
GCATGTCTTTTCTGGAAATCAGAGAGGTGCTGACCCCCATTCATCGAATTTGCGATGTCGCGCATCTTACCCTGGATACG
CACATTCATGGCCTCAGGATTGCGGAGCGATACAAATACAATGTTTGGGATGCAATGATTGTCGCATCGGCCCTGTTGGC
TGATTGCGCCGTTCTTTACACGGAGGATATGCACCACGGCCAGATCCTCGAAGACCGCTTGCAACTGATCAATCCATTTT
TGCGGGCCGACGAGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8919.15 Da
• Isoelectric Point: 8.7371

>AT39969 WP_009560865.1 NZ_AP025160:c2793638-2793405 [Acidithiobacillus ferrooxidans]
MQVSKWGNSLAVRIPTSVVEALGIKEGDDIEVEVVGERRFQIQRTPQAEELLARLRRYRGRLPADFRFDRLEAHGER

Nucleotide

• Length: 234 bp

>AT39969 NZ_AP025160:c2793638-2793405 [Acidithiobacillus ferrooxidans]
ATGCAGGTATCCAAGTGGGGCAACAGTCTGGCCGTCCGTATACCAACCTCCGTGGTAGAGGCGCTGGGTATCAAAGAGGG
TGACGACATTGAGGTTGAGGTCGTGGGCGAACGCCGTTTTCAGATTCAACGAACGCCTCAAGCGGAAGAGTTGTTGGCCA
GGTTAAGGCGTTATCGAGGGAGGCTCCCTGCCGATTTCAGGTTCGACCGGTTGGAAGCGCATGGCGAGCGGTAA

Structures

Toxin

• AlphaFold DB: B7J980

Antitoxin

• AlphaFold DB: B7J981