Detailed information of TA system TA039968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2687932..2688576
• Replicon: chromosome
• Accession: NZ_AP025160
• Organism: Acidithiobacillus ferrooxidans strain NFP31

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ANFP_RS13750
• Protein ID: WP_229129810.1
• Coordinates: 2687932..2688216 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7J8J7
• Locus tag: ANFP_RS13755
• Protein ID: WP_009566530.1
• Coordinates: 2688337..2688576 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANFP_RS13710 (ANFP_27570)	2683067..2683705	+	639	WP_225487201.1	lipoprotein insertase outer membrane protein LolB	-
ANFP_RS13715 (ANFP_27580)	2683702..2684547	+	846	WP_012537374.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
ANFP_RS13725 (ANFP_27590)	2684675..2685622	+	948	WP_009567132.1	ribose-phosphate pyrophosphokinase	-
ANFP_RS13735 (ANFP_27600)	2685975..2686337	-	363	Protein_2707	IS3 family transposase	-
ANFP_RS13740	2686456..2687367	-	912	WP_041646513.1	DUF2034 domain-containing protein	-
ANFP_RS13745 (ANFP_27630)	2687656..2687808	+	153	WP_009566528.1	hypothetical protein	-
ANFP_RS13750 (ANFP_27640)	2687932..2688216	-	285	WP_229129810.1	PIN domain-containing protein	Toxin
ANFP_RS13755 (ANFP_27650)	2688337..2688576	-	240	WP_009566530.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ANFP_RS13760 (ANFP_27660)	2688757..2689026	-	270	WP_012537379.1	hypothetical protein	-
ANFP_RS13765 (ANFP_27670)	2689245..2690126	-	882	WP_012607524.1	glutathione-dependent disulfide-bond oxidoreductase	-
ANFP_RS13770 (ANFP_27690)	2690775..2691560	-	786	WP_012537380.1	sulfite exporter TauE/SafE family protein	-
ANFP_RS13775 (ANFP_27700)	2691626..2691961	-	336	WP_041838695.1	thiamine pyrophosphate-dependent enzyme	-
ANFP_RS13780 (ANFP_27710)	2692036..2692863	-	828	WP_012536793.1	IS21-like element ISAfe9 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2685975..2686355	380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-84)

Antitoxin

• SpoVT-AbrB domain (10-55)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10161.72 Da
• Isoelectric Point: 6.7118

>T39968 WP_229129810.1 NZ_AP025160:c2688216-2687932 [Acidithiobacillus ferrooxidans]
VVCELRYGAAKKNSPRLTEQVNAVIAAMDVLPYDTPVDQAYAEIRQHLAAYGQPIGPNDLLIASHALAVKAILVSANVGE
FSRVPGLIVHNWLQ

Nucleotide

• Length: 285 bp

>T39968 NZ_AP025160:c2688216-2687932 [Acidithiobacillus ferrooxidans]
GTGGTTTGTGAATTACGGTATGGCGCAGCAAAGAAAAATTCTCCCCGACTCACAGAACAGGTGAACGCCGTTATCGCGGC
CATGGATGTGCTCCCCTATGACACGCCGGTCGATCAGGCTTATGCGGAAATCCGTCAACACCTTGCCGCCTACGGCCAGC
CCATTGGTCCGAATGACTTGCTTATTGCCTCCCATGCACTGGCAGTCAAGGCCATATTGGTGAGCGCCAATGTGGGCGAG
TTTTCCCGAGTGCCCGGGCTCATAGTGCACAATTGGCTGCAGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9405.66 Da
• Isoelectric Point: 4.7132

>AT39968 WP_009566530.1 NZ_AP025160:c2688576-2688337 [Acidithiobacillus ferrooxidans]
MHTERHVRFFRNGRNQAVRIPREFEMDAQEAIMRREDDRLIIEPVKRKGLLATLASLSALDEEFPDTDRDLLPLDDIEW

Nucleotide

• Length: 240 bp

>AT39968 NZ_AP025160:c2688576-2688337 [Acidithiobacillus ferrooxidans]
ATGCATACGGAACGCCATGTACGTTTTTTTCGGAATGGCCGCAATCAAGCGGTTCGCATTCCCCGTGAGTTCGAGATGGA
CGCGCAAGAGGCCATCATGCGGCGTGAGGATGATCGCTTGATTATCGAGCCCGTAAAACGCAAAGGGCTTCTGGCCACTC
TGGCGAGTCTTTCTGCCTTGGATGAGGAGTTCCCGGATACGGATCGCGATCTTCTCCCGCTGGATGACATCGAATGGTGA

Structures

Antitoxin

• AlphaFold DB: B7J8J7