Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/VapB(antitoxin) |
| Location | 2254224..2254792 | Replicon | chromosome |
| Accession | NZ_AP025160 | ||
| Organism | Acidithiobacillus ferrooxidans strain NFP31 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | A0A5J6VSB9 |
| Locus tag | ANFP_RS11465 | Protein ID | WP_012536619.1 |
| Coordinates | 2254418..2254792 (+) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | A0A2Z6IL32 |
| Locus tag | ANFP_RS11460 | Protein ID | WP_012536620.1 |
| Coordinates | 2254224..2254421 (+) | Length | 66 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ANFP_RS11415 (ANFP_22970) | 2249522..2250418 | + | 897 | WP_012536626.1 | hypothetical protein | - |
| ANFP_RS11420 (ANFP_22980) | 2250443..2250712 | + | 270 | WP_012536625.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | - |
| ANFP_RS11425 (ANFP_22990) | 2250699..2250833 | + | 135 | Protein_2250 | type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family | - |
| ANFP_RS16635 | 2250882..2251013 | - | 132 | WP_012607115.1 | hypothetical protein | - |
| ANFP_RS11430 | 2251436..2251657 | - | 222 | WP_009560828.1 | HU family DNA-binding protein | - |
| ANFP_RS11435 (ANFP_23010) | 2251666..2252019 | - | 354 | WP_009560827.1 | hypothetical protein | - |
| ANFP_RS11440 (ANFP_23020) | 2252031..2252306 | - | 276 | WP_012536624.1 | HU family DNA-binding protein | - |
| ANFP_RS11445 (ANFP_23030) | 2252373..2252696 | - | 324 | WP_012536623.1 | integration host factor subunit alpha | - |
| ANFP_RS11450 (ANFP_23040) | 2252693..2253367 | - | 675 | WP_012536622.1 | hypothetical protein | - |
| ANFP_RS11455 (ANFP_23050) | 2253717..2253878 | + | 162 | WP_012536621.1 | hypothetical protein | - |
| ANFP_RS11460 (ANFP_23060) | 2254224..2254421 | + | 198 | WP_012536620.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| ANFP_RS11465 (ANFP_23070) | 2254418..2254792 | + | 375 | WP_012536619.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| ANFP_RS16640 | 2254717..2255139 | + | 423 | WP_225981991.1 | N-6 DNA methylase | - |
| ANFP_RS11475 (ANFP_23090) | 2255194..2257992 | + | 2799 | WP_012536617.1 | class I SAM-dependent DNA methyltransferase | - |
| ANFP_RS11480 (ANFP_23100) | 2257989..2258648 | + | 660 | WP_012536616.1 | putative DNA binding domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | - | 2092361..2280399 | 188038 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 13683.71 Da Isoelectric Point: 6.3157
>T39967 WP_012536619.1 NZ_AP025160:2254418-2254792 [Acidithiobacillus ferrooxidans]
MILVDTSVWIDHFRSGDAHLSTLLDHAMVQTHDFIIGEIACGNLKNRQQTLYLLSGLPRCTATSPEETLFFIGRHSLMGR
GIGYIDASILASAMLVQANLWSRDRRLMALATELGCAYSPTSSE
MILVDTSVWIDHFRSGDAHLSTLLDHAMVQTHDFIIGEIACGNLKNRQQTLYLLSGLPRCTATSPEETLFFIGRHSLMGR
GIGYIDASILASAMLVQANLWSRDRRLMALATELGCAYSPTSSE
Download Length: 375 bp
>T39967 NZ_AP025160:2254418-2254792 [Acidithiobacillus ferrooxidans]
TTGATTCTGGTCGATACCTCCGTCTGGATCGACCATTTCCGCTCTGGAGATGCACACCTCTCTACTCTGCTTGATCATGC
CATGGTGCAAACGCACGATTTCATCATCGGAGAAATCGCTTGCGGGAATCTCAAAAATCGGCAGCAGACCTTATATCTGC
TCTCAGGCTTACCCCGATGCACCGCCACCTCTCCAGAAGAAACCCTGTTTTTTATTGGGCGTCATAGCCTGATGGGACGC
GGTATAGGCTATATTGACGCGAGCATCCTAGCCTCCGCCATGCTCGTGCAGGCCAATTTATGGTCCCGCGACAGACGTCT
CATGGCTCTGGCTACCGAACTTGGCTGCGCGTACTCGCCGACGTCATCCGAATAG
TTGATTCTGGTCGATACCTCCGTCTGGATCGACCATTTCCGCTCTGGAGATGCACACCTCTCTACTCTGCTTGATCATGC
CATGGTGCAAACGCACGATTTCATCATCGGAGAAATCGCTTGCGGGAATCTCAAAAATCGGCAGCAGACCTTATATCTGC
TCTCAGGCTTACCCCGATGCACCGCCACCTCTCCAGAAGAAACCCTGTTTTTTATTGGGCGTCATAGCCTGATGGGACGC
GGTATAGGCTATATTGACGCGAGCATCCTAGCCTCCGCCATGCTCGTGCAGGCCAATTTATGGTCCCGCGACAGACGTCT
CATGGCTCTGGCTACCGAACTTGGCTGCGCGTACTCGCCGACGTCATCCGAATAG
Antitoxin
Download Length: 66 a.a. Molecular weight: 7266.37 Da Isoelectric Point: 9.4896
>AT39967 WP_012536620.1 NZ_AP025160:2254224-2254421 [Acidithiobacillus ferrooxidans]
MRTTINLDDELLAQAKTLCGVEDRGALLKEALRALIERESARRLARLGGTEPQLQNIPRRREPAA
MRTTINLDDELLAQAKTLCGVEDRGALLKEALRALIERESARRLARLGGTEPQLQNIPRRREPAA
Download Length: 198 bp
>AT39967 NZ_AP025160:2254224-2254421 [Acidithiobacillus ferrooxidans]
ATGCGAACGACAATCAACCTGGATGATGAGCTCCTGGCCCAAGCCAAAACGCTCTGTGGCGTAGAAGACCGTGGCGCCCT
CTTAAAAGAGGCCCTGCGTGCTTTGATTGAACGGGAAAGTGCCCGTCGCCTCGCTCGCTTGGGTGGAACAGAACCGCAAT
TACAGAACATTCCTCGCAGACGAGAGCCTGCCGCTTGA
ATGCGAACGACAATCAACCTGGATGATGAGCTCCTGGCCCAAGCCAAAACGCTCTGTGGCGTAGAAGACCGTGGCGCCCT
CTTAAAAGAGGCCCTGCGTGCTTTGATTGAACGGGAAAGTGCCCGTCGCCTCGCTCGCTTGGGTGGAACAGAACCGCAAT
TACAGAACATTCCTCGCAGACGAGAGCCTGCCGCTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5J6VSB9 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6IL32 |