Detailed information of TA system TA039966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2224482..2224931
• Replicon: chromosome
• Accession: NZ_AP025160
• Organism: Acidithiobacillus ferrooxidans strain NFP31

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: ANFP_RS11275
• Protein ID: WP_012536654.1
• Coordinates: 2224785..2224931 (-)
• Length: 49 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ANFP_RS11270
• Protein ID: WP_012536655.1
• Coordinates: 2224482..2224781 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANFP_RS11240 (ANFP_22580)	2220338..2220715	-	378	WP_012536661.1	VirB3 family type IV secretion system protein	-
ANFP_RS11245 (ANFP_22590)	2220719..2221105	-	387	WP_012536660.1	TrbC/VirB2 family protein	-
ANFP_RS11250 (ANFP_22600)	2221310..2222101	+	792	WP_012536659.1	hypothetical protein	-
ANFP_RS11255 (ANFP_22620)	2222498..2223466	-	969	WP_012536657.1	P-type conjugative transfer ATPase TrbB	-
ANFP_RS11260 (ANFP_22630)	2223463..2223915	-	453	WP_012536656.1	hypothetical protein	-
ANFP_RS11265 (ANFP_22640)	2224068..2224373	-	306	WP_146235838.1	hypothetical protein	-
ANFP_RS11270 (ANFP_22650)	2224482..2224781	-	300	WP_012536655.1	putative addiction module antidote protein	Antitoxin
ANFP_RS11275 (ANFP_22660)	2224785..2224931	-	147	WP_012536654.1	addiction module killer protein	Toxin
ANFP_RS11280 (ANFP_22670)	2225363..2225506	-	144	WP_012536652.1	hypothetical protein	-
ANFP_RS11285 (ANFP_22680)	2225614..2225946	+	333	WP_012536651.1	hypothetical protein	-
ANFP_RS11290 (ANFP_22690)	2225937..2226284	+	348	WP_012536650.1	IS66 family insertion sequence element accessory protein TnpB	-
ANFP_RS11295 (ANFP_22700)	2226334..2227971	+	1638	WP_012536649.1	IS66 family transposase	-
ANFP_RS11300 (ANFP_22710)	2228016..2228210	+	195	WP_012536648.1	hypothetical protein	-
ANFP_RS11305 (ANFP_22720)	2228627..2229394	-	768	WP_012536647.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2092361..2280399	188038

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-46)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-92)

Sequences

Toxin

Protein

• Length: 49 a.a.
• Molecular weight: 5323.25 Da
• Isoelectric Point: 10.0955

>T39966 WP_012536654.1 NZ_AP025160:c2224931-2224785 [Acidithiobacillus ferrooxidans]
MRIHYGPGYRVYFKRFGLELVVLLAGGDKSTQSADIALALKLARQLEG

Nucleotide

• Length: 147 bp

>T39966 NZ_AP025160:c2224931-2224785 [Acidithiobacillus ferrooxidans]
ATGCGTATCCATTACGGTCCCGGCTATCGGGTGTACTTCAAGCGGTTTGGCCTGGAACTCGTCGTGCTTCTGGCCGGAGG
CGACAAATCCACGCAATCGGCCGACATTGCCCTAGCATTGAAGTTGGCACGACAACTGGAGGGGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10699.15 Da
• Isoelectric Point: 5.5858

>AT39966 WP_012536655.1 NZ_AP025160:c2224781-2224482 [Acidithiobacillus ferrooxidans]
MEKLELHTWDSAEHLKTEEDIAAYLDACFEEAGDDAAFIARALGTVARARGMTRLAHDAGMGRESLYKALSGEGNPSFAT
ILKVAKALGVRIRAEALHV

Nucleotide

• Length: 300 bp

>AT39966 NZ_AP025160:c2224781-2224482 [Acidithiobacillus ferrooxidans]
ATGGAAAAATTGGAATTGCACACATGGGACAGTGCCGAGCACCTGAAAACCGAAGAGGACATTGCGGCTTATCTCGACGC
CTGTTTTGAAGAGGCGGGGGATGACGCGGCCTTCATCGCCAGGGCGCTGGGTACCGTTGCCCGCGCGCGCGGCATGACCC
GGCTGGCCCATGACGCCGGCATGGGCAGGGAAAGCCTCTACAAAGCCCTTTCCGGAGAAGGCAACCCCAGTTTTGCCACC
ATTCTCAAGGTAGCCAAAGCCTTGGGTGTGCGCATTCGCGCCGAAGCTCTGCACGTCTGA