Detailed information of TA system TA039965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2154867..2155269
• Replicon: chromosome
• Accession: NZ_AP025160
• Organism: Acidithiobacillus ferrooxidans strain NFP31

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: -
• Locus tag: ANFP_RS10935
• Protein ID: WP_012536712.1
• Coordinates: 2154867..2155073 (-)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: B7JB38
• Locus tag: ANFP_RS10940
• Protein ID: WP_009569339.1
• Coordinates: 2155075..2155269 (-)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANFP_RS10930 (ANFP_21940)	2150721..2153216	-	2496	WP_049756718.1	diguanylate cyclase	-
ANFP_RS10935 (ANFP_21980)	2154867..2155073	-	207	WP_012536712.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ANFP_RS10940 (ANFP_21990)	2155075..2155269	-	195	WP_009569339.1	hypothetical protein	Antitoxin
ANFP_RS10945 (ANFP_22000)	2155379..2157865	-	2487	WP_012536711.1	type IV secretory system conjugative DNA transfer family protein	-
ANFP_RS10950 (ANFP_22010)	2157876..2158532	-	657	WP_012536710.1	lytic transglycosylase domain-containing protein	-
ANFP_RS10955 (ANFP_22020)	2158529..2158831	-	303	WP_012536709.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2092361..2280399	188038

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-62)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7857.11 Da
• Isoelectric Point: 8.0355

>T39965 WP_012536712.1 NZ_AP025160:c2155073-2154867 [Acidithiobacillus ferrooxidans]
MLPIIWRASARDDLANIIRYIANENPPTARRMKCLLEESVLPTAEHPYLYRISDRVPGLREIVAHQTT

Nucleotide

• Length: 207 bp

>T39965 NZ_AP025160:c2155073-2154867 [Acidithiobacillus ferrooxidans]
TTGTTACCCATTATCTGGCGGGCCTCGGCCCGCGATGACTTGGCGAATATCATTCGCTACATCGCCAACGAGAATCCGCC
AACGGCGCGGCGTATGAAATGCCTGCTGGAAGAGTCTGTCTTGCCTACTGCCGAACATCCCTACCTGTACCGCATCAGCG
ATCGCGTGCCGGGCTTGCGTGAGATCGTCGCGCACCAAACTACATAG

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7083.93 Da
• Isoelectric Point: 4.1598

>AT39965 WP_009569339.1 NZ_AP025160:c2155269-2155075 [Acidithiobacillus ferrooxidans]
MNAVLPSIISEFETAEQEASYTAWLRTKVASSLADQRPSIPHDEVMAEMDAIIAEAETSAQRKF

Nucleotide

• Length: 195 bp

>AT39965 NZ_AP025160:c2155269-2155075 [Acidithiobacillus ferrooxidans]
ATGAATGCCGTTTTACCTTCTATCATCTCCGAGTTTGAGACCGCCGAGCAGGAGGCCAGCTATACTGCGTGGCTGCGTAC
AAAGGTCGCGTCGAGCTTGGCTGATCAGCGACCCAGTATTCCGCATGACGAGGTGATGGCCGAAATGGACGCGATCATCG
CGGAAGCGGAAACCTCTGCTCAAAGGAAATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2Z6IL66