Detailed information of TA system TA039964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1876443..1877080
• Replicon: chromosome
• Accession: NZ_AP025160
• Organism: Acidithiobacillus ferrooxidans strain NFP31

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ANFP_RS09610
• Protein ID: WP_012536995.1
• Coordinates: 1876443..1876853 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7J4Y7
• Locus tag: ANFP_RS09615
• Protein ID: WP_009567455.1
• Coordinates: 1876850..1877080 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANFP_RS09590 (ANFP_19240)	1873023..1874090	-	1068	WP_225487051.1	phytoene/squalene synthase family protein	-
ANFP_RS09595 (ANFP_19250)	1874225..1875259	-	1035	WP_012536992.1	NAD(P)H-dependent glycerol-3-phosphate dehydrogenase	-
ANFP_RS09600 (ANFP_19260)	1875263..1875712	-	450	WP_012536993.1	protein-export chaperone SecB	-
ANFP_RS09610 (ANFP_19270)	1876443..1876853	-	411	WP_012536995.1	tRNA(fMet)-specific endonuclease VapC	Toxin
ANFP_RS09615 (ANFP_19280)	1876850..1877080	-	231	WP_009567455.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
ANFP_RS16620	1877232..1877354	+	123	WP_257246597.1	hypothetical protein	-
ANFP_RS09620 (ANFP_19290)	1877383..1878903	-	1521	WP_012536996.1	aldehyde dehydrogenase family protein	-
ANFP_RS09625 (ANFP_19300)	1879206..1880918	+	1713	WP_009566247.1	proline--tRNA ligase	-
ANFP_RS09630 (ANFP_19310)	1880905..1881387	+	483	WP_009566245.1	universal stress protein	-
ANFP_RS09635 (ANFP_19320)	1881400..1882023	-	624	WP_225487052.1	ABC-type transport auxiliary lipoprotein family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15375.79 Da
• Isoelectric Point: 8.0756

>T39964 WP_012536995.1 NZ_AP025160:c1876853-1876443 [Acidithiobacillus ferrooxidans]
VIKYLLDTNICIFTIKNKPPEIREQFRQHHGQMAISTVTLMELIYGAEKSQVPERNLAEVEGFAARLEVLEFGSEAAKHT
GQLRAELAKIGKPIGPYDQMIAGHARSLGLFVVTNNLREFERVPGLRVMDWQSSRL

Nucleotide

• Length: 411 bp

>T39964 NZ_AP025160:c1876853-1876443 [Acidithiobacillus ferrooxidans]
GTGATCAAATACCTGCTGGATACCAATATCTGCATCTTTACTATCAAGAACAAGCCACCGGAGATCCGCGAACAGTTCCG
TCAGCACCATGGGCAAATGGCTATCAGCACCGTCACCTTGATGGAGTTGATCTATGGCGCGGAGAAATCACAGGTACCGG
AACGCAACCTGGCCGAAGTTGAAGGGTTCGCCGCCAGGCTGGAAGTGCTCGAATTCGGGAGCGAAGCCGCCAAGCATACT
GGGCAGCTCCGCGCAGAACTGGCCAAGATCGGCAAACCCATCGGGCCTTATGACCAGATGATCGCAGGCCACGCCAGAAG
CCTGGGATTGTTTGTCGTGACCAACAACTTGCGGGAATTCGAGCGCGTTCCGGGATTGCGGGTCATGGATTGGCAGAGCT
CACGCCTGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8573.77 Da
• Isoelectric Point: 5.0508

>AT39964 WP_009567455.1 NZ_AP025160:c1877080-1876850 [Acidithiobacillus ferrooxidans]
MEHATVFQINRSQAIRLPKSIAFPADVKRVEVVAIGRMRILVPAGESWESWFDGEGVSADFMESREQPAGQFREPL

Nucleotide

• Length: 231 bp

>AT39964 NZ_AP025160:c1877080-1876850 [Acidithiobacillus ferrooxidans]
ATGGAGCATGCCACTGTTTTTCAGATCAACCGTAGCCAGGCGATACGTCTGCCCAAGTCGATAGCCTTTCCGGCAGATGT
GAAGCGCGTAGAGGTGGTCGCCATAGGCCGCATGCGCATTCTGGTTCCTGCCGGAGAGTCCTGGGAGAGCTGGTTCGATG
GAGAAGGGGTCAGCGCCGACTTCATGGAAAGCCGTGAGCAACCCGCCGGCCAGTTCCGGGAACCGTTGTGA

Structures

Antitoxin

• AlphaFold DB: B7J4Y7