Detailed information of TA system TA039963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1524100..1524774
• Replicon: chromosome
• Accession: NZ_AP025160
• Organism: Acidithiobacillus ferrooxidans strain NFP31

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B7JBN6
• Locus tag: ANFP_RS07925
• Protein ID: WP_009564531.1
• Coordinates: 1524355..1524774 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7JBN5
• Locus tag: ANFP_RS07920
• Protein ID: WP_012536752.1
• Coordinates: 1524100..1524354 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANFP_RS07905 (ANFP_15880)	1519775..1520737	-	963	WP_012607230.1	glycosyltransferase family 2 protein	-
ANFP_RS07910 (ANFP_15900)	1520918..1522378	+	1461	WP_012536750.1	IMP dehydrogenase	-
ANFP_RS07915 (ANFP_15910)	1522382..1523926	+	1545	WP_012536751.1	glutamine-hydrolyzing GMP synthase	-
ANFP_RS07920 (ANFP_15920)	1524100..1524354	+	255	WP_012536752.1	plasmid stabilization protein	Antitoxin
ANFP_RS07925 (ANFP_15930)	1524355..1524774	+	420	WP_009564531.1	type II toxin-antitoxin system VapC family toxin	Toxin
ANFP_RS07930 (ANFP_15940)	1524771..1525004	+	234	WP_226833825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
ANFP_RS07935 (ANFP_15950)	1525004..1525159	+	156	WP_012536753.1	hypothetical protein	-
ANFP_RS07940 (ANFP_15960)	1525839..1527458	+	1620	WP_012536754.1	APC family permease	-
ANFP_RS07945 (ANFP_15970)	1527549..1528748	+	1200	WP_012536755.1	glutamate 5-kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14968.11 Da
• Isoelectric Point: 4.7051

>T39963 WP_009564531.1 NZ_AP025160:1524355-1524774 [Acidithiobacillus ferrooxidans]
MIVLDTNVVSEAMKPEPDAAVRAWLNEQAAETLYLSSVTLAELLFGIRALPAGKCKNMLDRALTELLELFKDRVLPFDTD
AARHYADLAVTAKNGGRGFPTPDGYIAAVAASRGFIVASRDTSPYEAAGVTVINPWKDA

Nucleotide

• Length: 420 bp

>T39963 NZ_AP025160:1524355-1524774 [Acidithiobacillus ferrooxidans]
ATGATCGTCCTGGATACCAACGTCGTTTCCGAGGCGATGAAGCCGGAGCCAGACGCGGCCGTGAGGGCTTGGCTGAACGA
ACAGGCGGCGGAAACCTTGTATCTGTCGAGCGTCACGCTGGCCGAGTTGCTGTTCGGCATCCGGGCGCTGCCGGCGGGCA
AGTGCAAGAACATGCTCGACCGCGCCCTGACCGAACTGCTGGAGCTGTTCAAGGATCGGGTGCTGCCCTTCGATACGGAT
GCAGCGCGCCACTATGCCGACCTGGCCGTGACGGCCAAGAACGGCGGGCGCGGCTTCCCGACGCCAGACGGCTACATCGC
GGCTGTTGCCGCATCGCGGGGCTTCATCGTGGCATCACGCGACACGTCGCCCTATGAAGCGGCCGGCGTGACCGTCATCA
ATCCCTGGAAGGATGCATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9225.67 Da
• Isoelectric Point: 10.7239

>AT39963 WP_012536752.1 NZ_AP025160:1524100-1524354 [Acidithiobacillus ferrooxidans]
MAILTVRNVPDDVHRALRVRAARHGRSTEAEVREILAAAVKPSSRVCMGEALAAIGRKIGLTNEDFAVFESVRDKTPAKP
LRFD

Nucleotide

• Length: 255 bp

>AT39963 NZ_AP025160:1524100-1524354 [Acidithiobacillus ferrooxidans]
ATGGCAATCCTGACTGTAAGAAATGTGCCGGATGACGTGCATCGCGCTTTGCGCGTGCGGGCGGCCCGGCACGGTCGCAG
CACGGAAGCGGAGGTGCGCGAGATTCTGGCGGCTGCGGTCAAACCTTCGAGCCGCGTGTGCATGGGGGAAGCCCTGGCCG
CCATCGGCCGCAAGATCGGTCTGACCAATGAAGATTTCGCCGTTTTCGAGAGTGTGCGCGACAAGACGCCGGCCAAACCG
TTGCGCTTCGACTGA

Structures

Toxin

• AlphaFold DB: B7JBN6

Antitoxin

• AlphaFold DB: B7JBN5