Detailed information of TA system TA039960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09812-MazE
• Location: 1354762..1355381
• Replicon: chromosome
• Accession: NZ_AP025160
• Organism: Acidithiobacillus ferrooxidans strain NFP31

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ANFP_RS07010
• Protein ID: WP_054608979.1
• Coordinates: 1354762..1355115 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A2W1K4M0
• Locus tag: ANFP_RS07015
• Protein ID: WP_054608978.1
• Coordinates: 1355112..1355381 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANFP_RS06985 (ANFP_14050)	1351299..1351706	-	408	WP_054608983.1	hypothetical protein	-
ANFP_RS06990 (ANFP_14060)	1351703..1352131	-	429	WP_146235806.1	hypothetical protein	-
ANFP_RS06995 (ANFP_14070)	1352173..1353582	-	1410	WP_055448960.1	hypothetical protein	-
ANFP_RS07000 (ANFP_14080)	1353852..1354259	-	408	WP_054608981.1	transcriptional regulator	-
ANFP_RS07005 (ANFP_14090)	1354259..1354549	-	291	WP_054608980.1	type II toxin-antitoxin system HigB family toxin	-
ANFP_RS07010 (ANFP_14100)	1354762..1355115	-	354	WP_054608979.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ANFP_RS07015 (ANFP_14110)	1355112..1355381	-	270	WP_054608978.1	hypothetical protein	Antitoxin
ANFP_RS07020 (ANFP_14120)	1355530..1355781	+	252	WP_054608977.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
ANFP_RS07030 (ANFP_14130)	1356239..1357375	+	1137	WP_012536490.1	FIST N-terminal domain-containing protein	-
ANFP_RS07035 (ANFP_14140)	1357454..1359211	+	1758	WP_012536491.1	thiol reductant ABC exporter subunit CydD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12842.63 Da
• Isoelectric Point: 7.8845

>T39960 WP_054608979.1 NZ_AP025160:c1355115-1354762 [Acidithiobacillus ferrooxidans]
MIGRKIPSPGDVYWINPNPTAGNEIRDRHRFVVITPTEINKLGLVTCVPITSGGNYARQNGLTVVIAGHDTTGVAVCNQM
RTFDLHARAGDVKYVETLDQQTTREIINRVTSIIDPA

Nucleotide

• Length: 354 bp

>T39960 NZ_AP025160:c1355115-1354762 [Acidithiobacillus ferrooxidans]
TTGATCGGACGGAAGATTCCATCGCCCGGCGACGTGTACTGGATCAATCCCAACCCAACAGCAGGAAACGAAATCCGGGA
CCGGCACAGGTTCGTGGTCATCACACCCACCGAGATCAACAAGCTGGGCCTGGTAACGTGTGTACCCATCACCAGCGGCG
GCAATTACGCCAGGCAGAACGGATTGACCGTGGTCATCGCGGGGCACGACACGACGGGAGTGGCCGTCTGCAATCAAATG
CGTACCTTCGACCTGCACGCCAGAGCGGGGGACGTGAAGTACGTGGAGACGTTGGACCAGCAAACCACGCGCGAAATCAT
CAACCGGGTAACCAGCATCATCGATCCAGCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9708.11 Da
• Isoelectric Point: 5.1053

>AT39960 WP_054608978.1 NZ_AP025160:c1355381-1355112 [Acidithiobacillus ferrooxidans]
MLVTRIRKQGGAAVVTLPAEVLRQLDAGIGEDLSIEVSQHTVIMRPARGERRRYNLEELLAGVTGQDMQILAGDTAWARE
GDPAGRELI

Nucleotide

• Length: 270 bp

>AT39960 NZ_AP025160:c1355381-1355112 [Acidithiobacillus ferrooxidans]
ATGTTGGTCACAAGGATTCGCAAGCAAGGCGGTGCGGCTGTGGTTACGCTCCCCGCGGAAGTGTTGAGGCAGTTGGATGC
CGGCATCGGTGAGGATTTGAGCATCGAGGTCTCCCAGCACACGGTTATCATGCGTCCAGCACGCGGTGAGCGACGGCGCT
ACAACCTGGAAGAATTGCTCGCTGGCGTCACCGGGCAGGACATGCAGATCCTCGCCGGTGATACCGCTTGGGCCAGAGAA
GGCGATCCCGCCGGGAGAGAGTTGATTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2W1K4M0