Detailed information of TA system TA039958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 1140708..1141233
• Replicon: chromosome
• Accession: NZ_AP025160
• Organism: Acidithiobacillus ferrooxidans strain NFP31

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: B7J6U7
• Locus tag: ANFP_RS05930
• Protein ID: WP_009564272.1
• Coordinates: 1140952..1141233 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B7J6U6
• Locus tag: ANFP_RS05925
• Protein ID: WP_009564270.1
• Coordinates: 1140708..1140962 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANFP_RS16580 (ANFP_11860)	1135755..1136426	-	672	WP_012536411.1	LysR family transcriptional regulator	-
ANFP_RS05885 (ANFP_11870)	1136582..1136776	-	195	WP_225487690.1	hypothetical protein	-
ANFP_RS05890 (ANFP_11880)	1136769..1137068	+	300	WP_226834093.1	SDR family oxidoreductase	-
ANFP_RS05895 (ANFP_11890)	1137237..1137905	+	669	WP_009561118.1	cupin domain-containing protein	-
ANFP_RS05900 (ANFP_11900)	1137902..1139065	+	1164	WP_012536412.1	FAD-dependent oxidoreductase	-
ANFP_RS05905	1139062..1139181	+	120	WP_009564254.1	short-chain dehydrogenase	-
ANFP_RS05910 (ANFP_11910)	1139174..1139464	+	291	WP_041645337.1	hypothetical protein	-
ANFP_RS05915 (ANFP_11920)	1139639..1139974	+	336	WP_012536413.1	shufflon-specific DNA recombinase	-
ANFP_RS05920 (ANFP_11930)	1140167..1140460	+	294	WP_012606678.1	YbjQ family protein	-
ANFP_RS05925 (ANFP_11940)	1140708..1140962	+	255	WP_009564270.1	antitoxin	Antitoxin
ANFP_RS05930 (ANFP_11950)	1140952..1141233	+	282	WP_009564272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ANFP_RS05940 (ANFP_11960)	1141776..1142999	-	1224	WP_012536416.1	class I SAM-dependent rRNA methyltransferase	-
ANFP_RS05945 (ANFP_11970)	1143154..1144206	+	1053	WP_012536417.1	S-methyl-5-thioribose-1-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10757.61 Da
• Isoelectric Point: 10.6855

>T39958 WP_009564272.1 NZ_AP025160:1140952-1141233 [Acidithiobacillus ferrooxidans]
MPYELTFHPSALKEWQALDHSVRAPFKKKLAERLVHPRVPSAQLHGHPDRYKIKLRSIGYRLIYQVMETEVQVLVLAVGK
REGSVAYHKAKGR

Nucleotide

• Length: 282 bp

>T39958 NZ_AP025160:1140952-1141233 [Acidithiobacillus ferrooxidans]
ATGCCTTATGAGCTCACTTTCCATCCCTCCGCCCTCAAAGAATGGCAGGCCTTGGACCATAGCGTCCGTGCCCCATTCAA
AAAGAAACTGGCAGAACGCTTAGTGCACCCACGCGTCCCTTCCGCTCAACTGCATGGCCACCCGGATCGCTATAAGATCA
AACTCCGCAGCATAGGCTATCGCTTGATCTACCAAGTGATGGAAACGGAAGTCCAAGTGTTGGTCCTGGCGGTAGGCAAG
CGGGAAGGCTCTGTCGCCTATCACAAGGCCAAAGGACGCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 8832.16 Da
• Isoelectric Point: 4.6615

>AT39958 WP_009564270.1 NZ_AP025160:1140708-1140962 [Acidithiobacillus ferrooxidans]
MTHVILADVAASVSELKKNPMGTVAAGEGATVAILNRNEPAFYCVPAATYAALLEHLENLELNALAESRLKDGQTPIKVS
LDAL

Nucleotide

• Length: 255 bp

>AT39958 NZ_AP025160:1140708-1140962 [Acidithiobacillus ferrooxidans]
ATGACCCACGTCATTCTTGCCGATGTTGCTGCCAGTGTTTCCGAACTCAAAAAGAACCCTATGGGAACCGTGGCCGCAGG
AGAAGGGGCTACTGTGGCCATTCTCAACCGCAACGAACCGGCTTTTTACTGTGTCCCAGCCGCTACCTATGCTGCGCTTC
TGGAACATCTGGAAAATTTGGAGCTGAACGCTTTGGCGGAAAGCCGTCTGAAGGATGGCCAAACCCCGATCAAGGTGTCG
CTGGATGCCTTATGA

Structures

Toxin

• AlphaFold DB: B7J6U7

Antitoxin

• AlphaFold DB: B7J6U6