Detailed information of TA system TA039957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 499852..500492
• Replicon: chromosome
• Accession: NZ_AP025160
• Organism: Acidithiobacillus ferrooxidans strain NFP31

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ANFP_RS02695
• Protein ID: WP_009567232.1
• Coordinates: 500082..500492 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: B7J4M0
• Locus tag: ANFP_RS02690
• Protein ID: WP_009567231.1
• Coordinates: 499852..500082 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANFP_RS02675 (ANFP_05280)	495730..496860	+	1131	WP_012536159.1	threonine synthase	-
ANFP_RS02680 (ANFP_05290)	496860..497825	+	966	WP_009567244.1	hypothetical protein	-
ANFP_RS02685 (ANFP_05300)	497826..499643	+	1818	WP_012536160.1	DHHA1 domain-containing protein	-
ANFP_RS02690 (ANFP_05310)	499852..500082	+	231	WP_009567231.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
ANFP_RS02695 (ANFP_05320)	500082..500492	+	411	WP_009567232.1	type II toxin-antitoxin system VapC family toxin	Toxin
ANFP_RS02700 (ANFP_05330)	500625..501723	+	1099	WP_012606567.1	peptide chain release factor 2	-
ANFP_RS02705 (ANFP_05340)	501763..503250	+	1488	WP_229129796.1	lysine--tRNA ligase	-
ANFP_RS02710 (ANFP_05350)	503247..504491	+	1245	WP_012536161.1	lipoprotein-releasing ABC transporter permease subunit	-
ANFP_RS02715 (ANFP_05360)	504484..505179	+	696	WP_009566877.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-43)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15278.46 Da
• Isoelectric Point: 7.3848

>T39957 WP_009567232.1 NZ_AP025160:500082-500492 [Acidithiobacillus ferrooxidans]
MTLRYLLDTNICIYIAKHHPPAVRERFVQHGADDLAMSVITLGELCWGAEKSQARDKALASIQRLQERIQVAPLTPQVGI
HYGQIRAALESGGRPIGNNDLWIAAHARAEGWIVVTNNEREFRRVEGLQVENWVTA

Nucleotide

• Length: 411 bp

>T39957 NZ_AP025160:500082-500492 [Acidithiobacillus ferrooxidans]
ATGACCTTGCGTTATCTGCTGGATACCAATATCTGCATTTATATTGCCAAACACCATCCGCCCGCCGTGCGGGAGCGTTT
TGTACAACATGGTGCCGATGATCTGGCGATGTCGGTAATCACCTTGGGTGAATTGTGTTGGGGTGCGGAAAAAAGTCAGG
CGCGTGACAAAGCGTTGGCCAGCATACAGCGCCTGCAAGAACGTATTCAGGTGGCCCCCCTCACCCCTCAGGTCGGCATC
CACTATGGCCAGATCCGCGCGGCTCTGGAAAGCGGCGGGCGGCCCATTGGCAATAATGATCTGTGGATCGCCGCACACGC
CCGCGCCGAAGGCTGGATCGTAGTTACCAACAATGAACGAGAGTTCCGACGGGTGGAAGGATTGCAAGTGGAAAACTGGG
TAACGGCATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8704.76 Da
• Isoelectric Point: 4.4217

>AT39957 WP_009567231.1 NZ_AP025160:499852-500082 [Acidithiobacillus ferrooxidans]
MAIARIFQSGNSQAVRLPKEFRFEVDQVEIFRRGNEIILREHPVDGTGVFDALADLPEDFMSDGRQDIPPQARENF

Nucleotide

• Length: 231 bp

>AT39957 NZ_AP025160:499852-500082 [Acidithiobacillus ferrooxidans]
ATGGCCATCGCACGCATCTTCCAATCAGGCAACAGTCAGGCCGTACGGTTGCCCAAAGAATTCCGCTTTGAGGTGGATCA
GGTGGAAATCTTCCGCCGCGGGAATGAGATTATTCTGCGGGAGCATCCAGTGGATGGTACAGGCGTTTTTGATGCCCTGG
CCGACCTTCCCGAAGACTTCATGTCCGACGGACGCCAGGATATTCCACCCCAGGCAAGGGAAAACTTTTGA

Structures

Antitoxin

• AlphaFold DB: B7J4M0