Detailed information of TA system TA039956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 173080..173699
• Replicon: chromosome
• Accession: NZ_AP025160
• Organism: Acidithiobacillus ferrooxidans strain NFP31

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A2W1KCH5
• Locus tag: ANFP_RS00840
• Protein ID: WP_031575563.1
• Coordinates: 173403..173699 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ANFP_RS00835
• Protein ID: WP_031575560.1
• Coordinates: 173080..173397 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANFP_RS00810 (ANFP_01680)	168610..169755	+	1146	WP_229129756.1	hypothetical protein	-
ANFP_RS00815 (ANFP_01690)	169837..170577	+	741	WP_054608922.1	P-type conjugative transfer protein TrbJ	-
ANFP_RS00820 (ANFP_01700)	170583..170843	+	261	WP_229129757.1	EexN family lipoprotein	-
ANFP_RS00825 (ANFP_01710)	170857..172551	+	1695	WP_054608923.1	P-type conjugative transfer protein TrbL	-
ANFP_RS00830 (ANFP_01720)	172622..173026	+	405	WP_031575557.1	hypothetical protein	-
ANFP_RS00835 (ANFP_01730)	173080..173397	-	318	WP_031575560.1	putative addiction module antidote protein	Antitoxin
ANFP_RS00840 (ANFP_01740)	173403..173699	-	297	WP_031575563.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ANFP_RS00845	173920..174324	+	405	WP_031575566.1	hypothetical protein	-
ANFP_RS00850	174317..175237	-	921	WP_146235851.1	hypothetical protein	-
ANFP_RS00855 (ANFP_01760)	175387..176055	+	669	WP_054608924.1	type IV secretion system protein	-
ANFP_RS00860 (ANFP_01770)	176068..177168	+	1101	WP_054608925.1	TrbG/VirB9 family P-type conjugative transfer protein	-
ANFP_RS00865 (ANFP_01780)	177170..178174	+	1005	WP_054608926.1	TrbG/VirB9 family P-type conjugative transfer protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (4-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11112.88 Da
• Isoelectric Point: 9.6531

>T39956 WP_031575563.1 NZ_AP025160:c173699-173403 [Acidithiobacillus ferrooxidans]
MLELIETNVFREWLEGLRDHQARARIQVRMRRLSLGNPGDVKPVGEGISEMRIDYGPGYRVYFMRRGPVVVALLCGGDKG
SQERDIALAKAIAAQWKE

Nucleotide

• Length: 297 bp

>T39956 NZ_AP025160:c173699-173403 [Acidithiobacillus ferrooxidans]
ATGCTTGAGCTCATAGAAACCAACGTGTTCAGGGAATGGCTCGAAGGTCTGCGCGACCACCAAGCCCGCGCAAGGATTCA
GGTGCGGATGCGCCGATTGAGCCTTGGGAACCCTGGAGACGTGAAGCCTGTAGGTGAAGGCATCTCGGAAATGCGGATTG
ACTACGGTCCTGGCTATCGCGTCTATTTCATGAGGCGCGGACCAGTTGTGGTGGCGCTTCTGTGCGGCGGAGACAAGGGG
AGCCAGGAACGGGACATTGCTTTGGCAAAAGCCATTGCAGCGCAATGGAAGGAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11253.69 Da
• Isoelectric Point: 6.2117

>AT39956 WP_031575560.1 NZ_AP025160:c173397-173080 [Acidithiobacillus ferrooxidans]
MEVQFRRYDTVNYLKTEDDIAHYLDAAMEDGDPALILAALGDVARARNLSQLARDAGMSRQGLDKALSGTGNPSLVTVLK
ITQALGLRLSFQPAHTSSHPSATGV

Nucleotide

• Length: 318 bp

>AT39956 NZ_AP025160:c173397-173080 [Acidithiobacillus ferrooxidans]
ATGGAAGTGCAATTCAGACGCTACGACACCGTGAATTACCTCAAGACTGAGGACGATATTGCGCACTATCTCGATGCTGC
GATGGAGGATGGCGACCCCGCGTTGATTCTTGCAGCGCTGGGAGACGTTGCCCGTGCCCGCAACCTCAGCCAGCTCGCCC
GCGACGCAGGCATGAGCCGCCAAGGATTGGACAAGGCCCTTTCCGGAACAGGGAACCCAAGCCTGGTAACGGTGCTGAAA
ATAACCCAGGCACTCGGCCTGCGGCTTTCCTTCCAACCAGCACACACAAGCAGTCACCCATCGGCTACAGGGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A2W1KCH5