Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 108472..109052 Replicon plasmid pSA4125
Accession NZ_AP025033
Organism Aureimonas sp. SA4125

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag Sa4125_RS24015 Protein ID WP_224008392.1
Coordinates 108472..108798 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag Sa4125_RS24020 Protein ID WP_224008395.1
Coordinates 108795..109052 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Sa4125_RS23990 (Sa4125_48000) 105812..106264 + 453 WP_224008590.1 ester cyclase -
Sa4125_RS23995 (Sa4125_48010) 106346..106696 - 351 WP_224008383.1 DUF2384 domain-containing protein -
Sa4125_RS24000 (Sa4125_48020) 107189..107611 + 423 Protein_104 hypothetical protein -
Sa4125_RS24005 (Sa4125_48030) 107753..107998 + 246 WP_224008386.1 type II toxin-antitoxin system VapB family antitoxin -
Sa4125_RS24010 (Sa4125_48040) 107998..108255 + 258 WP_224008389.1 type II toxin-antitoxin system VapC family toxin -
Sa4125_RS24015 (Sa4125_48050) 108472..108798 - 327 WP_224008392.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
Sa4125_RS24020 (Sa4125_48060) 108795..109052 - 258 WP_224008395.1 antitoxin Antitoxin
Sa4125_RS24025 (Sa4125_48070) 109371..110297 + 927 WP_224008399.1 zincin-like metallopeptidase domain-containing protein -
Sa4125_RS24030 (Sa4125_48080) 110849..111160 + 312 WP_224008401.1 DUF736 domain-containing protein -
Sa4125_RS24035 (Sa4125_48090) 111536..111673 + 138 WP_224008403.1 hypothetical protein -
Sa4125_RS24040 (Sa4125_48100) 111748..111987 - 240 WP_224008405.1 WGR domain-containing protein -
Sa4125_RS24045 (Sa4125_48110) 112337..113620 + 1284 WP_224008407.1 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..131777 131777
- flank IS/Tn - - 112352..113620 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-107)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11890.63 Da        Isoelectric Point: 7.2778

>T39924 WP_224008392.1 NZ_AP025033:c108798-108472 [Aureimonas sp. SA4125]
MKRGDVYIVDLEPTHGREQRGHRPVVIVSPDDFNQATNLPVIAPITNGGDFAHRIGFAVPLDGTQTTGVVRCDQPRVLDI
KARNGRKVETLPAAIMDEVLARIATIFY

Download         Length: 327 bp

>T39924 NZ_AP025033:c108798-108472 [Aureimonas sp. SA4125]
ATGAAGCGCGGCGACGTTTACATCGTCGATCTGGAACCGACCCACGGCCGTGAACAGCGCGGGCATCGCCCAGTCGTGAT
TGTCTCCCCCGACGATTTCAACCAGGCCACCAACCTGCCGGTCATCGCGCCGATCACGAACGGCGGCGACTTCGCTCACC
GCATCGGCTTTGCCGTCCCGCTCGACGGGACCCAAACTACCGGGGTGGTGCGCTGCGATCAGCCCCGCGTTCTCGACATC
AAAGCCCGCAACGGCCGCAAAGTGGAAACCCTTCCGGCGGCGATCATGGACGAGGTCTTGGCCAGGATTGCGACCATCTT
CTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9111.51 Da        Isoelectric Point: 4.9731

>AT39924 WP_224008395.1 NZ_AP025033:c109052-108795 [Aureimonas sp. SA4125]
MHTTNLRKVGGSIMLAVPPALLDVLHLSAGAKVGLAIDNGRLVVEPQARPRYTMAELLAASDYPQPQPAEEREWVDAPAV
GNELL

Download         Length: 258 bp

>AT39924 NZ_AP025033:c109052-108795 [Aureimonas sp. SA4125]
ATGCACACCACAAACCTTCGCAAGGTCGGCGGTTCGATCATGCTGGCCGTCCCGCCCGCACTGCTTGACGTTCTGCACCT
GTCCGCCGGCGCGAAGGTCGGGCTTGCCATCGATAACGGCCGTCTCGTGGTCGAGCCGCAGGCGCGGCCGCGCTATACGA
TGGCGGAGCTTCTGGCCGCGTCCGATTATCCCCAGCCGCAGCCTGCCGAAGAACGCGAATGGGTGGACGCACCAGCCGTG
GGCAACGAACTCCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References