Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 60889..61414 Replicon plasmid pNUITM-VR1_3
Accession NZ_AP025012
Organism Raoultella ornithinolytica strain NUITM-VR1

Toxin (Protein)


Gene name ccdB Uniprot ID A0A6S4YF27
Locus tag LDO46_RS30325 Protein ID WP_025714517.1
Coordinates 61109..61414 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag LDO46_RS30320 Protein ID WP_001568025.1
Coordinates 60889..61107 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO46_RS30310 (NUITMVR1_59820) 58571..59599 - 1029 WP_001568023.1 Abi family protein -
LDO46_RS30315 (NUITMVR1_59830) 59740..60309 - 570 WP_016162066.1 hypothetical protein -
LDO46_RS30320 (NUITMVR1_59850) 60889..61107 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LDO46_RS30325 (NUITMVR1_59860) 61109..61414 + 306 WP_025714517.1 type II toxin-antitoxin system toxin CcdB Toxin
LDO46_RS30330 (NUITMVR1_59870) 61583..61978 + 396 WP_017899885.1 hypothetical protein -
LDO46_RS30335 (NUITMVR1_59880) 62005..62319 + 315 WP_053389906.1 hypothetical protein -
LDO46_RS30340 (NUITMVR1_59890) 62330..63346 + 1017 WP_017899884.1 hypothetical protein -
LDO46_RS30345 (NUITMVR1_59900) 63544..64338 + 795 WP_004197635.1 site-specific integrase -
LDO46_RS30350 (NUITMVR1_59920) 64802..65104 - 303 WP_071571079.1 hypothetical protein -
LDO46_RS30355 (NUITMVR1_59930) 65101..65727 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3'')-Ib / aph(6)-Id / qnrS1 / blaNDM-1 htpB 1..139908 139908


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11562.25 Da        Isoelectric Point: 5.6906

>T39905 WP_025714517.1 NZ_AP025012:61109-61414 [Raoultella ornithinolytica]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T39905 NZ_AP025012:61109-61414 [Raoultella ornithinolytica]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT39905 WP_001568025.1 NZ_AP025012:60889-61107 [Raoultella ornithinolytica]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT39905 NZ_AP025012:60889-61107 [Raoultella ornithinolytica]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6S4YF27

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References