Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/ElaA-DUF1778 |
| Location | 234879..235618 | Replicon | plasmid pNUITM-VR1_2 |
| Accession | NZ_AP025011 | ||
| Organism | Raoultella ornithinolytica strain NUITM-VR1 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | - |
| Locus tag | LDO46_RS29895 | Protein ID | WP_076752153.1 |
| Coordinates | 234879..235364 (-) | Length | 162 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | A0A5E1AWX8 |
| Locus tag | LDO46_RS29900 | Protein ID | WP_012540086.1 |
| Coordinates | 235352..235618 (-) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LDO46_RS29865 (NUITMVR1_58950) | 232000..232416 | - | 417 | WP_076752145.1 | helix-turn-helix domain-containing protein | - |
| LDO46_RS29870 (NUITMVR1_58960) | 232413..232724 | - | 312 | WP_076752146.1 | type II toxin-antitoxin system HigB family toxin | - |
| LDO46_RS29875 (NUITMVR1_58970) | 232874..233086 | - | 213 | WP_076752148.1 | hypothetical protein | - |
| LDO46_RS29880 (NUITMVR1_58980) | 233194..233520 | - | 327 | WP_076752150.1 | hypothetical protein | - |
| LDO46_RS29885 | 234379..234495 | - | 117 | WP_223176086.1 | type I toxin-antitoxin system Hok family toxin | - |
| LDO46_RS29890 | 234443..234595 | - | 153 | WP_223526259.1 | DUF5431 family protein | - |
| LDO46_RS29895 (NUITMVR1_59010) | 234879..235364 | - | 486 | WP_076752153.1 | GNAT family N-acetyltransferase | Toxin |
| LDO46_RS29900 (NUITMVR1_59020) | 235352..235618 | - | 267 | WP_012540086.1 | DUF1778 domain-containing protein | Antitoxin |
| LDO46_RS29905 (NUITMVR1_59030) | 235919..236242 | + | 324 | WP_013087171.1 | metalloregulator ArsR/SmtB family transcription factor | - |
| LDO46_RS29910 (NUITMVR1_59040) | 236244..236957 | + | 714 | WP_013087170.1 | arsenical resistance protein ArsH | - |
| LDO46_RS29915 (NUITMVR1_59050) | 236966..237511 | + | 546 | WP_047365773.1 | sigma-70 family RNA polymerase sigma factor | - |
| LDO46_RS29920 (NUITMVR1_59060) | 237587..237949 | + | 363 | WP_013087168.1 | arsenic metallochaperone ArsD family protein | - |
| LDO46_RS29925 (NUITMVR1_59070) | 237976..239727 | + | 1752 | WP_187420813.1 | arsenical pump-driving ATPase | - |
| LDO46_RS29930 (NUITMVR1_59080) | 239852..240388 | + | 537 | WP_013087166.1 | GNAT family N-acetyltransferase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | mcr-10 / qnrS1 / aph(6)-Id / aph(3'')-Ib | - | 1..261835 | 261835 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 162 a.a. Molecular weight: 17838.68 Da Isoelectric Point: 10.0748
>T39904 WP_076752153.1 NZ_AP025011:c235364-234879 [Raoultella ornithinolytica]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSRTQQRTLFLKLP
Q
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSRTQQRTLFLKLP
Q
Download Length: 486 bp
>T39904 NZ_AP025011:c235364-234879 [Raoultella ornithinolytica]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACGAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACGAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG
Antitoxin
Download Length: 89 a.a. Molecular weight: 10076.47 Da Isoelectric Point: 4.3721
>AT39904 WP_012540086.1 NZ_AP025011:c235618-235352 [Raoultella ornithinolytica]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV
Download Length: 267 bp
>AT39904 NZ_AP025011:c235618-235352 [Raoultella ornithinolytica]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|