Detailed information of TA system TA039902

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 251838..252591
• Replicon: plasmid pNUITM-VR1_1
• Accession: NZ_AP025010
• Organism: Raoultella ornithinolytica strain NUITM-VR1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: R8WN26
• Locus tag: LDO46_RS28045
• Protein ID: WP_016154457.1
• Coordinates: 252214..252591 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: LDO46_RS28040
• Protein ID: WP_162838704.1
• Coordinates: 251838..252164 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO46_RS27980 (NUITMVR1_54950)	246896..247162	+	267	WP_009652486.1	hypothetical protein	-
LDO46_RS27985 (NUITMVR1_54960)	247175..247486	+	312	WP_009652410.1	hypothetical protein	-
LDO46_RS27990 (NUITMVR1_54970)	247533..248003	+	471	WP_009652431.1	hypothetical protein	-
LDO46_RS27995 (NUITMVR1_54980)	248052..248564	+	513	WP_009652395.1	DUF4234 domain-containing protein	-
LDO46_RS28000 (NUITMVR1_54990)	248647..248868	+	222	WP_009652443.1	hypothetical protein	-
LDO46_RS28005 (NUITMVR1_55000)	248924..249157	+	234	WP_009652474.1	DUF905 domain-containing protein	-
LDO46_RS28010 (NUITMVR1_55010)	249261..250079	+	819	WP_009652424.1	DUF932 domain-containing protein	-
LDO46_RS28015 (NUITMVR1_55020)	250079..250333	+	255	WP_009652519.1	hypothetical protein	-
LDO46_RS28020 (NUITMVR1_55030)	250350..250823	+	474	WP_009652453.1	antirestriction protein	-
LDO46_RS28025 (NUITMVR1_55040)	250834..251052	+	219	WP_009652503.1	hypothetical protein	-
LDO46_RS28030 (NUITMVR1_55050)	251064..251546	+	483	WP_016154455.1	DNA repair protein RadC	-
LDO46_RS28035 (NUITMVR1_55060)	251560..251781	+	222	WP_009652392.1	DUF987 domain-containing protein	-
LDO46_RS28040 (NUITMVR1_55070)	251838..252164	+	327	WP_162838704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LDO46_RS28045 (NUITMVR1_55080)	252214..252591	+	378	WP_016154457.1	TA system toxin CbtA family protein	Toxin
LDO46_RS28050 (NUITMVR1_55090)	252588..253079	+	492	WP_009652438.1	DUF5983 family protein	-
LDO46_RS28055 (NUITMVR1_55100)	253109..253312	+	204	WP_009652505.1	DUF957 domain-containing protein	-
LDO46_RS28060 (NUITMVR1_55120)	253393..254238	+	846	Protein_285	DUF4942 domain-containing protein	-
LDO46_RS28065 (NUITMVR1_55140)	254438..255469	+	1032	WP_223175620.1	hypothetical protein	-
LDO46_RS28070 (NUITMVR1_55150)	255472..255945	+	474	WP_009652494.1	ribbon-helix-helix protein, CopG family	-
LDO46_RS28075 (NUITMVR1_55160)	256265..256903	+	639	WP_009652437.1	VWA domain-containing protein	-
LDO46_RS28080 (NUITMVR1_55170)	256928..257569	+	642	WP_009652489.1	TerD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrS1 / blaNDM-1 / blaTEM-1B / blaLAP-2 / aac(3)-IId / catA2 / tet(D)	-	1..378524	378524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14168.19 Da
• Isoelectric Point: 9.5224

>T39902 WP_016154457.1 NZ_AP025010:252214-252591 [Raoultella ornithinolytica]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T39902 NZ_AP025010:252214-252591 [Raoultella ornithinolytica]
ATGCAAACACAACCTGTTCCTCCGAAACGGGAGGTTTCGCCACGCCCGTCACCCGTGGCGATCTGGCAGCGCCTCCTGAG
TCACCTGCTGGACCGTCACTACGGTCTGACGCTCAACGACACGCCATTTGGCAAAGATGGTGTGATCCAAGAGCATATCG
ATGCCGGTATCTCACTGTGCGACGCGGTGAATTTCATCGTGGAGAAATACGACCTGGTACGCACTGATCGTCGCGGTTTT
AACGCGGAAACTCAGTCGCCGCTTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGCTGATGACCCGTCA
TAGCTACAGGGCCGTTACCGACATCACCACCGGCAAATACCGTGAGGTGCAACCATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12079.85 Da
• Isoelectric Point: 6.6283

>AT39902 WP_162838704.1 NZ_AP025010:251838-252164 [Raoultella ornithinolytica]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT39902 NZ_AP025010:251838-252164 [Raoultella ornithinolytica]
ATCGCCGCCCCTTGGTGGGGGCTTAAACGCGATATCACCCCCTGTTTTGGCGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCATTACCTGAACGACCGCGCCAGCATCACCGGCACCTTCAGCGACGCGGATTTACGCCATCTCGACCAGGCATTCCCGC
TACTGCTGAAGCAACTGGAGCTGATGCTGCTTTCCGGTGAGCTGAATCCCCGTCATCAGCACTGCGTCACGCTGTATGCA
AAAGGGCTGATATGCGAGGCCGACAGCCTCGGCTCGCACGGGTACGTCTATCTCGCAATTTACCCGACACCTGCAACAAC
AGAATAA

Structures

Toxin

• AlphaFold DB: R8WN26