Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4953130..4953706 Replicon chromosome
Accession NZ_AP025009
Organism Raoultella ornithinolytica strain NUITM-VR1

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag LDO46_RS23405 Protein ID WP_118860181.1
Coordinates 4953419..4953706 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID -
Locus tag LDO46_RS23400 Protein ID WP_118860180.1
Coordinates 4953130..4953432 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO46_RS23385 (NUITMVR1_46100) 4949068..4949403 + 336 WP_044348942.1 endoribonuclease SymE -
LDO46_RS23390 (NUITMVR1_46110) 4949635..4951707 + 2073 WP_064357554.1 AAA family ATPase -
LDO46_RS23395 (NUITMVR1_46120) 4951707..4953029 + 1323 WP_044348938.1 mcrBC 5-methylcytosine restriction system component family protein -
LDO46_RS23400 (NUITMVR1_46130) 4953130..4953432 - 303 WP_118860180.1 BrnA antitoxin family protein Antitoxin
LDO46_RS23405 (NUITMVR1_46140) 4953419..4953706 - 288 WP_118860181.1 BrnT family toxin Toxin
LDO46_RS23410 (NUITMVR1_46150) 4953823..4954257 - 435 WP_118860182.1 FosA family fosfomycin resistance glutathione transferase -
LDO46_RS23415 (NUITMVR1_46160) 4954251..4955159 - 909 WP_004857513.1 LysR family transcriptional regulator -
LDO46_RS23420 (NUITMVR1_46170) 4955247..4956029 + 783 WP_104896592.1 NAD(P)H-dependent oxidoreductase -
LDO46_RS23425 (NUITMVR1_46180) 4956177..4956755 + 579 WP_113991322.1 TetR/AcrR family transcriptional regulator -
LDO46_RS23430 (NUITMVR1_46190) 4956899..4957711 + 813 WP_004857492.1 winged helix-turn-helix domain-containing protein -
LDO46_RS23435 (NUITMVR1_46200) 4957708..4958226 + 519 WP_015585229.1 FidL-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11157.65 Da        Isoelectric Point: 7.5236

>T39901 WP_118860181.1 NZ_AP025009:c4953706-4953419 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRHERYENGEYRWQTIGLVHGIIVILVVHSVRFESGAEVIRIIS
ARKADKKERSRYEHG

Download         Length: 288 bp

>T39901 NZ_AP025009:c4953706-4953419 [Raoultella ornithinolytica]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAGCAAGCAATCTAAGAAAACATGGGATTCGCTTCGAGGAGGCGGT
ACTGGTTTTTGACGATCCGCAGCATCTTTCCCGACATGAACGCTATGAAAATGGTGAGTATCGATGGCAAACCATCGGCC
TCGTTCATGGAATTATCGTTATTCTTGTGGTGCATTCGGTCCGCTTTGAAAGCGGGGCTGAGGTGATTCGTATCATCAGC
GCCCGGAAGGCGGACAAAAAAGAGAGGAGTCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 7.0167

>AT39901 WP_118860180.1 NZ_AP025009:c4953432-4953130 [Raoultella ornithinolytica]
MSMVKHKRDNPTALTAQREDELKALARKSDDDIDYSDIPSSDDEPWSEAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMNRDLGEK

Download         Length: 303 bp

>AT39901 NZ_AP025009:c4953432-4953130 [Raoultella ornithinolytica]
ATGAGCATGGTTAAACATAAACGCGATAACCCGACAGCGCTTACCGCTCAGCGTGAAGACGAACTCAAAGCGCTGGCGAG
AAAATCAGATGATGACATTGATTACAGCGATATCCCCTCTTCGGACGATGAGCCGTGGTCAGAGGCGGTTCGTGGTAAAT
TTTATCGGCCATTAAAAACGCAGGCCTCTGTGCGCATCGATGCAGATGTGATGGAGTGGCTTAAACGCCCGGGAAAAGGC
TATCAAACGCGGCTGAATGCGATTTTAAGAGAAGCGATGAACCGCGATCTGGGTGAGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References